Objective: To observe the effect of serum containing Banxia Xiexin Tang and its different combinations with other herbs on growth and proliferation of Bone Mesenchymal Stem Cells (BMSCs) in gastric carcinoma microenvironment. Method: To BMSCs were routinely cultured as blank group. In this study

a co-culture system of BMSCs and gastric carcinoma cell line BCG-823 was established by using transwell chamber as model group. Groups of serum containing of Banxia Xiexin Tang and its different combinations with other herbs (Quanfang group

Xinkai group

Kujiang group and Ganbu group) were added with the corresponding drug-containing serums at the concentration of 10%. The phase-contrast microscope was used to the morphology of BMSCs cell

methylthiazolyldiphenyl-tetrazolium bromide(MTT) method was adopted to observe the proliferation rate of BMSCs

flow cytometry (FCM) was applied to test BMSCs cell cycle

c-Myc and expression of telomerase reverse transcriptase (TERT) protein were detected by Western blot

fluorescence Real-time PCR test was used to detect c-Myc and TERT mRNA expressions. Result: Quanfang group

Xinkai group

Kujiang group and Ganbu group medicated serum at 4

5

6

7 d had a significant inhibitory effect on the proliferation of BMSCs

Quanfang group was superior to Ganbu group (P<0.05)

the detection results at the 7th day show that Quanfang group

Xinkai group

Kujiang group and Ganbu group medicated serum can decrease c-Myc and TERT expressions

elevate G1 phase cell proportion

and reduce the proportion of S phase cells

and Quanfang group was superior to Ganbu group (P<0.05). Conclusion: The serum containing Banxia Xiexin Tang and its different combinations with other herbs inhibit the abnormal proliferation of BMSCs in gastric carcinoma microenvironment

the overall prescription of Banxia Xiexin Tang shows the best compatibility.