四君子汤含药血清上调c-Myc表达促进小肠上皮细胞迁移和增殖

宋厚盼; 李如意; 魏艳霞; 李鑫; 余黄合; 袁振仪; 蔡雄; 黄惠勇; 刘翠玲

doi:10.13422/j.cnki.syfjx.2016220154

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四君子汤含药血清上调c-Myc表达促进小肠上皮细胞迁移和增殖

药理 | 更新时间：2024-01-04

- 四君子汤含药血清上调c-Myc表达促进小肠上皮细胞迁移和增殖
- Effect of Sijunzi Tang in Promoting Migration and Proliferation of Small Intestinal Epithelial Cells by Up-Regulating c-Myc Expression
- 中国实验方剂学杂志 2016年22卷第22期页码：154-159
- 作者机构：
- 作者简介：
- 基金信息：
  
  湖南省科技重大专项（2014FJ1007）;湖南省教育厅优秀青年项目（15B179）;湖南中医药大学博士科研启动项目（1001-017）;湖南中医药大学方剂学省级重点学科开放基金项目（2015-02）;湖南中医药大学中西医诊断学国家重点学科开放基金项目（2015ZYZD19）
- DOI：10.13422/j.cnki.syfjx.2016220154
  中图分类号： R285
- 纸质出版日期：2016
- 稿件说明：
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宋厚盼, 李如意, 魏艳霞, 等. 四君子汤含药血清上调c-Myc表达促进小肠上皮细胞迁移和增殖[J]. 中国实验方剂学杂志, 2016,22(22):154-159.

SONG Hou-pan, LI Ru-yi, WEI Yan-xia, et al. Effect of Sijunzi Tang in Promoting Migration and Proliferation of Small Intestinal Epithelial Cells by Up-Regulating c-Myc Expression[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(22): 154-159.
宋厚盼, 李如意, 魏艳霞, 等. 四君子汤含药血清上调c-Myc表达促进小肠上皮细胞迁移和增殖[J]. 中国实验方剂学杂志, 2016,22(22):154-159. DOI： 10.13422/j.cnki.syfjx.2016220154.

SONG Hou-pan, LI Ru-yi, WEI Yan-xia, et al. Effect of Sijunzi Tang in Promoting Migration and Proliferation of Small Intestinal Epithelial Cells by Up-Regulating c-Myc Expression[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(22): 154-159. DOI： 10.13422/j.cnki.syfjx.2016220154.

摘要

目的：考察四君子汤含药血清（SJZ）对小肠上皮（IEC-6）细胞损伤后迁移、增殖及c-核蛋白类基因（c-Myc）mRNA和蛋白表达的影响，以探讨四君子汤促进胃肠黏膜损伤修复的作用机制。方法：采用大鼠制备四君子汤含药血清，Tips划痕法建立IEC-6细胞迁移模型，观察四君子汤药物血清对细胞迁移的影响；实时细胞分析仪（RTCA）检测四君子汤含药血清对IEC-6细胞增殖的影响；实时荧光定量PCR（Real-time PCR）法检测c-Myc mRNA表达；蛋白免疫印迹（Western blot）法检测c-Myc蛋白表达。结果：中体积浓度（10%）和高体积浓度（20%）的四君子汤含药血清可促进IEC-6细胞迁移（P<0.01）；细胞损伤后12 h，中体积浓度（10%）和高体积浓度（20%）的四君子汤含药血清可促进IEC-6细胞增殖（P<0.01）；细胞损伤后24 h和36 h，低（5%），中（10%），高（20%）体积浓度的四君子汤含药血清均可促进IEC-6细胞增殖（P<0.05，P<0.01）；进一步研究发现，中体积浓度（10%）和高体积浓度（20%）的四君子汤含药血清可提高与胃肠黏膜损伤修复密切相关的c-Myc mRNA和蛋白表达（P<0.01）。结论：四君子汤修复胃肠黏膜屏障损伤，促进溃疡愈合的作用可能与其提高c-Myc mRNA和蛋白表达，促进黏膜上皮细胞的迁移和增殖有关。

Abstract

Objective: To study the effect of Sijunzi Tang (SJZT)-containing serum on cell migration and proliferation

c-Myc mRNA and protein expression of injured intestinal epithelial (IEC-6) cell

so as to explore the mechanism of SJZT in promoting gastrointestinal mucosal injury repair. Method: Rats were selected to prepare SJZT-containing serum

and IEC-6 cell migration model was established by tips scratch method

so as to observe the effect of SJZT on cell migration. Real-time cell analyzer (RTCA) was used to determine the effect of SJZT-containing serum on IEC-6 cell proliferation

c-Myc mRNA and protein expressions were detected by Real-time PCR and Western blot analysis

respectively. Result: The 10% and 20% SJZT-containing serum stimulated IEC-6 cell migration 8 h after cell damage (P<0.01); 10% and 20% SJZT-containing serum increased IEC-6 cell proliferation 12 h after cell damage (P<0.01). 5%

10%

and 20% SJZT-containing serum enhanced IEC-6 proliferation both at 24

36 h after damage (P<0.05

P<0.01). Further studies showed that

10% and 20% SJZT-containing serum increased c-Myc mRNA and protein expressions that were closely correlated with gastrointestinal mucosal injury repair (P<0.01). Conclusion: Sijunzi Tang-containing serum can repair the injury of gastrointestinal mucosal barrier

the mechanism may be related to its effect in improving mucosal c-Myc mRNA and protein expressions and promoting IEC-6 cell migration and proliferation.

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