不同剂量配伍对黄芪-当归中5种化学成分的影响

唐蓉; 张依人; 陈叶童; 蒋龙波; 邓常清; 邹龙

doi:10.13422/j.cnki.syfjx.2016230001

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不同剂量配伍对黄芪-当归中5种化学成分的影响

药剂与炮制 | 更新时间：2024-01-04

- 不同剂量配伍对黄芪-当归中5种化学成分的影响
- Effect of Different Compatibility on Five Chemical Components in Astragali Radix-Angelicae Sinensis Radix
- 中国实验方剂学杂志 2016年22卷第23期页码：1-5
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81473581）
- DOI：10.13422/j.cnki.syfjx.2016230001
  中图分类号： R284.1
- 纸质出版日期：2016
- 稿件说明：
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唐蓉, 张依人, 陈叶童, 等. 不同剂量配伍对黄芪-当归中5种化学成分的影响[J]. 中国实验方剂学杂志, 2016,22(23):1-5.

TANG Rong, ZHANG Yi-ren, CHEN Ye-tong, et al. Effect of Different Compatibility on Five Chemical Components in Astragali Radix-Angelicae Sinensis Radix[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(23): 1-5.
唐蓉, 张依人, 陈叶童, 等. 不同剂量配伍对黄芪-当归中5种化学成分的影响[J]. 中国实验方剂学杂志, 2016,22(23):1-5. DOI： 10.13422/j.cnki.syfjx.2016230001.

TANG Rong, ZHANG Yi-ren, CHEN Ye-tong, et al. Effect of Different Compatibility on Five Chemical Components in Astragali Radix-Angelicae Sinensis Radix[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(23): 1-5. DOI： 10.13422/j.cnki.syfjx.2016230001.

摘要

目的：采用UPLC-MS研究黄芪-当归不同剂量配伍药液及小肠吸收液中5种化学成分（阿魏酸、毛蕊异黄酮苷、毛蕊异黄酮、芒柄花素、黄芪甲苷）的含量变化，为二者的临床配伍用药提供参考。方法：利用水提法制备黄芪-当归不同剂量配伍的药液，运用大鼠外翻肠囊法进行药物吸收试验制备药物的小肠吸收液，通过UPLC-MS测定药液和小肠吸收液中5种成分的含量，流动相0.1%甲酸水溶液-乙腈梯度洗脱，流速0.4 mL·min-1，进样量1 μL，毛细管电压4 kV。结果：黄芪-当归以10：1和5：1配伍时，5种成分的溶出量大多与理论值相近；当黄芪-当归以1：1和1：5配伍时，5种成分的溶出大多高于理论值。黄芪-当归10：1和5：1配伍时，相应质量浓度小肠吸收液中毛蕊异黄酮苷、毛蕊异黄酮、黄芪甲苷和阿魏酸吸收率大多数高于单用黄芪和单用当归；黄芪-当归1：1和1：5配伍时，相应质量浓度小肠吸收液中5种成分的吸收率大多数高于单用黄芪和单用当归。结论：黄芪-当归以1：1和1：5配伍时可促进药物中大多数成分的溶出，且黄芪和当归不同剂量配伍时可不同程度地促进大多数成分的吸收。

Abstract

Objective: To study on the change of five chemical components (ferulic acid

calycosin-7-O-β-D-glycoside

calycosin

formononetin and astragaloside Ⅳ) in solution and small intestine absorption liquid of Astragali Radix-Angelicae Sinensis Radix with different compatibility. Method: Decoction of Astragali Radix-Angelicae Sinensis Radix with different compatibility was prepared by water extraction method

the small intestinal absorption liquid was prepared by drug absorption experiment which was carried out with isolated everted intestinal method.The contents of five components in solution and small intestine absorption liquid were determined by UPLC-MS. Result: The dissolution of five components were mostly close to the theoretical values while Astragali Radix-Angelicae Sinensis Radix at 10:1 and 5:1

but most of them were larger than the theoretical values while Astragali Radix-Angelicae Sinensis Radix at 1:1 and 1:5 ratio.When Astragali Radix-Angelicae Sinensis Radix (10:1)

most of the absorption rate of calycosin-7-O-β-D-glycoside

calycosin

astragaloside Ⅳ and ferulic acid in small intestinal absorption liquid with the same concentration were higher than that of the single use of Astragali Radix and Angelicae Sinensis Radix.When Astragali Radix-Angelicae Sinensis Radix 5:1

most of the absorption rate of five components in small intestinal absorption liquid with the same concentration were higher than that of the single use of Astragali Radix-Angelicae Sinensis Radix. Conclusion: The dissolution of five components can be improved when Astragali Radix-Angelicae Sinensis Radix at 1:1 and 1:5

different compatibility of Astragali Radix and Angelicae Sinensis Radix can promote the absorption of five components in varying degrees.

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