响应曲面法优化毛果鱼藤总生物碱的提取工艺及其抑菌活性

杨立芳; 刘洪存; 支媛; 郭洋洋; 闫文超; 姜明国; 谭学才; 石灵芳

doi:10.13422/j.cnki.syfjx.2016230028

您当前的位置：

首页 >

文章列表页 >

响应曲面法优化毛果鱼藤总生物碱的提取工艺及其抑菌活性

药剂与炮制 | 更新时间：2024-01-04

- 响应曲面法优化毛果鱼藤总生物碱的提取工艺及其抑菌活性
- Optimization of Extraction Process and Antibacterial Activity of Total Alkaloids from Based on Response Surface Method
- 中国实验方剂学杂志 2016年22卷第23期页码：28-34
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81560713）;广西教育厅科学研究项目（YB2014101）
- DOI：10.13422/j.cnki.syfjx.2016230028
  中图分类号： R284.2
- 纸质出版日期：2016
- 稿件说明：
移动端阅览
杨立芳, 刘洪存, 支媛, 等. 响应曲面法优化毛果鱼藤总生物碱的提取工艺及其抑菌活性[J]. 中国实验方剂学杂志, 2016,22(23):28-34.

YANG Li-fang, LIU Hong-cun, ZHI Yuan, et al. Optimization of Extraction Process and Antibacterial Activity of Total Alkaloids from Based on Response Surface Method[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(23): 28-34.
杨立芳, 刘洪存, 支媛, 等. 响应曲面法优化毛果鱼藤总生物碱的提取工艺及其抑菌活性[J]. 中国实验方剂学杂志, 2016,22(23):28-34. DOI： 10.13422/j.cnki.syfjx.2016230028.

YANG Li-fang, LIU Hong-cun, ZHI Yuan, et al. Optimization of Extraction Process and Antibacterial Activity of Total Alkaloids from Based on Response Surface Method[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(23): 28-34. DOI： 10.13422/j.cnki.syfjx.2016230028.

摘要

目的：利用响应曲面分析法优化毛果鱼藤总生物碱的提取工艺，并检测其抑菌活性。方法：以毛果鱼藤为原料，采用微波提取法，设计单因素实验，分别考察了料液比、乙醇体积分数、提取时间、微波提取功率、提取次数5个单因素对毛果鱼藤总生物碱提取效果的影响；采用响应曲面法优化毛果鱼藤总生物碱的提取工艺，提高总生物碱的提取效率，并考察总生物碱的体外抑菌作用。结果：毛果鱼藤总生物碱的最佳提取工艺条件为微波功率400 W，提取温度为70℃，料液比为1：23，乙醇体积分数为70%，提取时间为23 min，在此条件下，以盐酸小檗碱计，总生物碱提取率为0.107 7 mg·g-1；抑菌实验表明，毛果鱼藤总生物碱对金黄色葡萄球菌有一定的抑菌作用。结论：微波法提取生物碱工艺方法科学、稳定，且生物碱具有良好的抑菌作用。

Abstract

Objective: To optimize the extraction conditions of total alkaloids from Derris eriocarpa by response surface method

and detect their antibacterial activity. Method: Microwave extraction was used for one-single factor experiment of D. eriocarpa to investigate the effects of solid-liquid ratio

ethanol volume fraction

extraction time

microwave power

and number of extraction times on the extraction of total alkaloids from D. eriocarpa. Response surface method (RSM) was employed to optimize the extraction process

improve the extraction efficiency of total alkaloids

and investigate the in vitro antibacterial effect of total alkaloids. Result: The optimal extraction parameters were as follows:microwave extraction power of 400 W

extraction temperature of 70℃

solid-liquid ratio of 1:23

extraction time of 23 min and ethanol volume fraction of 70%. The experiment extraction yield of the total alkaloids was 0.107 7 mg·g-1 under these optimal conditions measured by berberine hydrochloride. The antibacterial activity data suggested that the total alkaloids had a certain inhibitory effect on the Staphylococcus aureus. Conclusion: The microwave extraction method for total alkaloids from D. eriocarpa was efficient and stable

and the total alkaloids showed good antibacterial effect.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

南天竹总生物碱对三氧化二砷减毒作用及其LC-MS分析

小檗皮总生物碱提取物的大孔树脂纯化工艺与质量标准考察

咳喘平片提取工艺的实验研究

荷叶中生物碱提取及纯化工艺研究