Objective: In the previous study

278 strains of endophytic fungi were separated from Rumex gmelini in different origins. These endophytic fungi were taken as the research object to screen out endophytic fungi that could produce nepodin-8-O-β-D-glucopyranoside and polydatin through fermentation. Method: HPLC was used to detect secondary metabolites of endophytic fungi

nepodin-8-O-β-D-glucopyranoside and polydatin were used as reference to screen out endophytic fungi that could produce nepodin-8-O-β-D-glucopyranoside and polydatin

and determine their content. Result: Eleven strains of endophytic fungi whose secondary metabolites containing nepodin-8-O-β-D-glucopyranoside were found

meanwhile 5 strains could produce polydatin. Among them

fungi tq-s1 had the highest mass fraction of nepodin-8-O-β-D-glucopyranoside (530 μg·g-1) and polydatin (224 μg·g-1) in its secondary metabolites. Conclusion: Endogenous fungal species and formation of their secondary metabolites were affected by geographical location. Further studies shall be conducted on the development and utilization of high-yield strain tq-s1 of both nepodin-8-O-β-D-glucopyranoside and polydatin.