Objective: To establish a quantitative analysis of multi-components by single marker (QAMS) method and realize quality control of Ligustri Lucidi Fructus. Method: HPLC method was used in this paper for determining the contents of specnuezhenide

nuezhenoside

oleuropein

salidroside and oleonuezhenide. With specnuezhenide as a reference

the relative correction factors (fi

s) of other four constituents were used as a quantitative parameter and the values of relative retention time (γi

s) were used as a qualitative parameter. Result: All five components were well separated. The results of system suitability test and methodology met the requirement of content determination. All the calibration curves showed good linearity in corresponding concentration ranges

with the correlation coefficients ≥0.999 5.The recoveries of these target compounds were in the range of 96.8%-105.0% (RSD≤3%). RSD of fi

s and γi

s were respectively less than 5% and 2% within the determination range. The results calculated with the proposed single marker method were consistent with those obtained by the external standard method

showing no significant differences between these two methods. Conclusion: The proposed QAMS method was proven to be operable and rapid with reliable results

which can be used for quality control of Ligustri Lucidi Fructus with multi-target ingredients.