指纹图谱法及紫外光谱法比较鸦胆子油与多种食用油

田金苗; 孙苓苓; 王怡君; 郭汉文; 孔令峰; 郝延军

doi:10.13422/j.cnki.syfjx.2016230078

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指纹图谱法及紫外光谱法比较鸦胆子油与多种食用油

化学与分析 | 更新时间：2024-01-04

- 指纹图谱法及紫外光谱法比较鸦胆子油与多种食用油
- Comparison Between Bruceae Fructus Oil and Other Cooking Oils by Fingerprint and UV Spectrum
- 中国实验方剂学杂志 2016年22卷第23期页码：78-82
- 作者机构：
- 作者简介：
- 基金信息：
  
  辽宁省科学技术计划项目（2014020166）
- DOI：10.13422/j.cnki.syfjx.2016230078
  中图分类号： TS225
- 纸质出版日期：2016
- 稿件说明：
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田金苗, 孙苓苓, 王怡君, 等. 指纹图谱法及紫外光谱法比较鸦胆子油与多种食用油[J]. 中国实验方剂学杂志, 2016,22(23):78-82.

TIAN Jin-miao, SUN Ling-ling, WANG Yi-jun, et al. Comparison Between Bruceae Fructus Oil and Other Cooking Oils by Fingerprint and UV Spectrum[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(23): 78-82.
田金苗, 孙苓苓, 王怡君, 等. 指纹图谱法及紫外光谱法比较鸦胆子油与多种食用油[J]. 中国实验方剂学杂志, 2016,22(23):78-82. DOI： 10.13422/j.cnki.syfjx.2016230078.

TIAN Jin-miao, SUN Ling-ling, WANG Yi-jun, et al. Comparison Between Bruceae Fructus Oil and Other Cooking Oils by Fingerprint and UV Spectrum[J]. Chinese journal of experimental traditional medical formulae, 2016, 22(23): 78-82. DOI： 10.13422/j.cnki.syfjx.2016230078.

摘要

目的：建立鸦胆子油的HPLC-ELSD指纹图谱和紫外光谱分析方法，并与多种食用油进行比较。方法：运用SSI高效液相色谱仪和奥泰2000蒸发光检测器建立指纹图谱，采用Waters C18色谱柱（4.6 mm×200 mm，5 μm），流动相乙腈-二氯甲烷（60：40），流速1.2 mL·min-1，检测温度85℃，柱温40℃，进样量15 μL。运用PERSEE紫外分光光度计TU-1950，进行紫外全波长扫描。结果：方法的精密度、重复性与稳定性良好，相对保留时间和相对峰面积的RSD均<3.0%，各峰分离度良好，指纹信息完整。结论：在此方法的基础上，建立了鸦胆子油及11种植物油的HPLC-ELSD指纹图谱和紫外光谱分析方法，为鸦胆子油质量控制和市场监管提供了重要依据。

Abstract

Objective: To establish HPLC-ELSD fingerprint and UV spectral analysis method for Bruceae Fructus oil

and compare with a variety of other cooking oils. Method: SSI HPLC and Alltech 2000 ESLD were used to establish the fingerprint on Waters C18 analytical column (4.6 mm×200 mm

5 μm)

with acetonitrile-dichloromethane (60:40) as the mobile phase at the flow rate of 1.2 mL·min-1. The detection temperature was 85℃; column temperature was 40℃

and injection volume was 15 μL. PERSEE UV-VIS spectrophotometer TU-1950 was used for UV full-wavelength scanning. Result: The results showed good precision

repeatability and stability for this method

and RSD of the relative retention time and relative peak area were less than 3.0%. Various peaks were well separated and the fingerprint information was complete. Conclusion: On the basis of this method

HPLC-ELSD fingerprints of Bruceae Fructus oil and 11 kinds of cooking oil and UV spectral analysis method were established

providing important basis for the quality control and market supervision of Bruceae Fructus oil.

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