Objective: To establish the HPLC fingerprints of Shenlingcao oral liquid

evaluate the quality status of Shenlingcao oral liquid. Method: The HPLC separation was preformed on a DIKMA SpursilTM C18-EP column(4.6 mm×250 mm

5 μm)

gradient eluted with mobile phase was acetonitrile(A)-0.1%phosphoric(B) at the flow rate of 1 mL·min-1

the detection wavelength was 260 nm.Through the fingerprint

the effective components and principal component of the raw medicine were identified

with the raw medicine and the effective components of Shenlingcao oral liquid in the prescription for the contrast. The quality of the Shenlingcao oral was judged by the similarity of the fingerprints. Result: The average RSD of retention time were all less than 0.5%

and the average RSD of peak area was less than 4% for precision

repeatability and stability of the fingerprints. The main component of the Shenlingcao oral liquid prescription herbs

as well as the active ingredient can find in the Shenlingcao oral liquid fingerprint. The oral liquid washing samples' similarity of 54 batches Shenlingcao is greater than 0.95

the alcohol washing samples' similarity is greater than 0.98. Conclusion: The oral liquid contains the main effective components of the medicinal herbs. Shenlingcao oral liquid was stable safe and effective. Fingerprint method can provide more comprehensive information for the the Shenlingcao oral solution quality evaluation.