Objective: To select the best semi-bionic extraction method of Zhizi Bopi Tang

and provide the scientific basis for extracting process of Zhizi Bopi decoction. Method: The herbs in the prescription were arranged in different combinations based on ‘Jun(A)

Chen(B)

Zuo(C) and Shi(D)’ principle. There were 5 combinations in total

and semi-bionic extraction method was used for extraction. The levels of gardenoside

berberine hydrochloride

glycyrrhetinic acid

total alkaloids and extracts with molecular weight≤1 000 Da were used as the indexes. The comprehensive evaluation values were acquired by using weighted summation of the five indexes after standardized treatment. Those with larger comprehensive evaluation values were better than others. Result: The comprehensive evaluation value Y was B+AC >A+B+C >C+AB >ABC >A+BC respectively for the 5 indexes

and B+AC was the biggest value. Conclusion: The semi-bionic extraction method of Zhizi Bopi Tang is decocting Cortex Phellodendri Chinensis solely first

then decocting Gardeniae Fructus and Glycyrrhizae Radix together

and finally combining the above extract Tang.