Objective: Using the UHPLC-ESI-Q-TOF-MS/MS to analyze and identify the the main components in the water extract of Violae Herba. Method: The separation was performed on UHPLC Welch C18 column (2.1 mm×100 mm

1.7 μm)

with a mobile phase using water containing 0.1% formic acid (A) and acetonitrile (B) for gradient elution

the flow rate was 0.35 mL·min-1. Electrospray ion (ESI) source were applied for the analysis under the positive/negative ion mode

and the running time was 30 min. Result: Based on the standards of MS/MS and compared with the reference results

32 compounds

containing coumarins

flavonoids and organic acids were identified. Conclusion: The efficient separation ability of UHPLC and high sensitive detection of MS are used in this study

which will provide the evidences for evaluating the quality of Violae Herba

stabilizing the curative effect in clinic

and explaining the mechanism.