Objective: The concents of aconitine

mesaconitine and hypaconitine in rat plasma were determined by UPLC-MS/MS after oral administration of Shiwuwei Rupeng pills

and pharmacokinetics of these three alkaloids in rats was investigated. Method: The separation was carried out on Welch UPLC XB-C18 column (2.0 mm×50 mm

1.7 μm)

mobile phase was acetonitrile-0.1% formic acid in water for gradient elution

flow rate was 0.35 mL·min-1.Electrospray ionization (ESI) source was applied and operated in the positive ion mode. Result: The linear ranges of aconitine

mesaconitine and hypaconitine were 126.56-10 125

18.75-1 500

16.32-1 305.5 ng·L-1

respectively.The main pharmacokinetic parameters of aconitine

mesaconitine and hypaconitine were obtained as follows:Cmax of (0.75±0.14)

(0.23±0.03)

(0.17±0.02) μg·L-1

t1/2 of (2.93±1.72)

(3.02±1.60)

(3.70±2.63) h

AUC0-t of (5.03±1.37)

(1.51±0.26)

(1.14±0.21) h·μg·L-1. Conclusion: This method is rapid

accurate and sensitive

and it can be used for pharmacokinetic study of such alkaloids

which provides pharmacokinetic evidence for in vivo study of Shiwuwei Rupeng pills.