Objective: To investigate the effect of Tongluo Tangtai power (TLTTP) on JNK signaling pathway in rats of diabetic peripheral neuropathy (DPN). Method: Totally 70 GK rats were selected to set up the rat model of spontaneous DPN induced by high fat diet. the 50 successfully modeled DPN rats were randomly divided into five groups

namely model group

TLTTP high-dose group

TLTTP group

TLTTP Low-dose group

and metformin+methycobal group

with 10 rats in each group; another 10 male GK rats were selected in normal group. All of the rats were detected for the expressions of p-JNK and Caspase-3 protein

JNK and Insulin-like growth factor 1(IGF-1) mRNA

sciatic nerve conduction velocity

and the levels of tumor necrosis factor-α(TNF-α)

interleukins-6(IL-6)

C-reactive protein(CRP)

myelin basic protein(MBP) in serum after 4 weeks of intragastric administration. Result: Compared with the normal group

the model group showed significant increase in p-JNK and Caspase-3 proteins

JNK mRNA expression

and serum TNF-α

IL-6

CRP and MBP levels

and significant decrease in IGF-1 mRNA expression (P<0.05); TLTTP could down-regulate the expression of p-JNK

Caspase3

JNK mRNA

increase the expression of IGF-1 mRNA

significantly improve sciatic nerve conduction velocity

and significantly reduce the levels of TNF-α

IL-6

CRP

MBP in serum. Conclusion: TLTTP may prevent the development of DPN by inhibiting JNK signaling pathway

mitigating ell apoptosis

IR

inflammation reaction and abnormal expression of downstream target genes.