Objective: To investigate effects of Astragali Radix extracts on glucose absorption in Caco-2 cell and its relevant regulation mechanism. Method: All extracts of Astragali Radix were extracted by using systematical solvent separation method. Except for the blank group

Caco-2 cells were treated with Astragali Radix extracts

and then divided into four extraction groups (ethanol

petroleum ether

ethyl acetate

and n-butanol). The glucose absorption was detected by using the fluorescent D-glucose analog

with 2-deoxyglucose (2-NBDG) as the probe indicator. After the efficient extracts for promoting glucose absorption in Caco-2 cell was extracted from Astragali Radix

its effective concentration was studied. The expression of sodium-dependent glucose transporter 1 (SGLT1) and glucose transporter 2 (GLUT2) were detected by Western blot. Result: Astragali Radix n-butanol extract could improve glucose absorption in Caco-2 cell. Compared with control group

Astragali Radix n-butanol extract 100

150 mg·L-1 group showed significant increase in glucose content in Caco-2 cells (P<0.05

P<0.01). N-butyl alcohol extracts of Astragali Radix could up-regulate GLUT2 and SGLT1 of Caco-2 cells (P<0.05

P<0.01). Conclusion: Astragali Radix n-butanol extract can promote the glucose absorption in Caco-2 cells. Its mechanism of improving glucose absorption may be correlated with regulation of SGLT1 and GLUT2.