Objective: To optimize ingredients in Jiedu Huoxue Chinese medicine compound (geniposide

ligustrazine and glycyrrhizic acid) for inhibiting excessive immune response. Method: In the study

uniform design was adopted to design doses of geniposide

ligustrazine and glycyrrhizic acid

the liquichip technique was used to detect the effect of different compatibility level on expression of 23 kinds of cytokines in RAW264.7 inflammation model induced by lipopolysaccharide (LPS)

with the comprehensive weight method

the traditional Chinese medicine component optimization software and the improved multiple linear regression algorithm were used to produce the regression equation and the optimal dose ratio of geniposide

ligustrazine and glycyrrhizic acid

through analyzing the dose-effect relationship among the three components and the cytokine inhibition rate. Result: When the ratio of geniposide

ligustrazine and glycyrrhizic acid was 1:20:19

the combination had the best anti-inflammatory effects in RAW264.7 inflammation model induced by LPS

its predicted and experimentally obtained values of comprehensive inhibition rate were 43.3%and 28.3%. Conclusion: The combination of uniform design and cell models in pharmacodynamics is an effective way to optimize the proportion of Chinese medicinal components.