Objective: Using a simple and reliable HPLC-DAD method

for Qixue Shuangbu tincture (QST) of fingerprint analysis and simultaneous determination of 10 bioactive constituents to carry out quality control. Method: Through optimal extraction

separation and selection of analytical conditions

Merck C18 chromatographic column was adopted at wavelength of 203 nm

with acetonitrile and 0.1% phosphoric acid as mobile phase. Result: According to fingerprint analysis on 10 batches of samples

32 peaks were adopted as the characteristic peaks and evaluation indexes. The similarity of the 10 batches of samples was high

and the determination of content of ten active components was stable and reliable. Conclusion: The multicomponent determination method combined with the chromatographic fingerprint analysis is a practical and meaningful method

and can be used for overall quality control of QST.