Objective: A liquid chromatography-tandem mass spectrometric method (LC-MS/MS) was developed and validated for simultaneous determination of albiflorin

paeoniflorin

liquiritin

baicalin

berberine hydrochloride

wogonoside

glycyrrhizic acid

baicalein and wogonin in rat plasma with icariin as the internal standard. Method: Concentrations of the target analytes in plasma were determined by LC-MS/MS

chromatographic separation was accomplished on a Promosil C18 column (4.6 mm×250 mm

5 μm) with a mobile phase consisting of acetonitrile and 0.1%formic acid aqueous solution for gradient elution.Detection was performed in the multiple reaction monitoring mode with a positive ionization mode.Two time segments were performed in a single HPLC run

the first 7 minutes just scanned ions of albiflorin

paeoniflorin and liquiritin

the second time segment scanned ions of the remaining analytes. Result: All calibration curves showed good linearity (r>0.998) over a wide concentration range for all analytes.RSDs of intra-day and inter-day precisions were all within 15%and the relative recovery of accuracy were in the range of 90.58%to 108.67%.The peak time (tmax) of these nine components were (0.25±0)

(0.25±0)

(0.25±0)

(0.25±0)

(1.00±0.10)

(6.33±0.82)

(1.00±0.11)

(0.25±0)

(3.12±1.15) h

respectively;the half life (t1/2) of them were (0.21±0.02)

(6.72±2.10)

(2.70±0.36)

(6.90±1.55)

(5.16±0.95)

(4.34±1.02)

(4.74±2.52)

(4.79±1.48)

(5.78±2.41) h

respectively;the peak concentration (Cmax) of them were (3.08±0.94)

(10.01±2.17)

(18.73±2.84)

(36.18±7.57)

(50.76±10.61)

(793.70±214.08)

(54.73±18.01)

(207.35±27.58)

(50.79±5.95) μg·L-1

respectively. Conclusion: The validated method is successfully applied to pharmacokinetics of nine components in rats after oral administration of Qingqing granules.