Objective: To investigate the anti-hepatitis B virus(HBV) effects of sophora alkaloids combined with entecavir

and observe its effect on p38 MAPK and sodium taurocholote cotransporting poly peptide(NTCP) expression levels. Method: Entecavir-resistant hepatitis HBV replication ell lines HepG2.A64 were used as the models

and then CPE and CCK-8 were used respectively to investigate the cytotoxicity in case of oxymatrine

matrine

sophoridine

sophocarpine or nucleoside drug entecavir as well as combined use of entecavir and any above sophora alkaloids. The levels of HbsAg

HBV DNA replication

p38 and NTCP mRNA expression

as well as p38 and p-p38 protein expression levels were detected by ELISA

Real-time PCR and Western blot. Result: As compared with medication alone

HBsAg secretion

HBV DNA replication were significantly inhibited 72 h after combined use of oxymatrine and entecavir

and NTCP expression levels and p38 phosphorylation levels were significantly reduced. Conclusion: The combined use of oxymatrine and entecavir could achieve the anti-HBV effect by down-regulating the expression of NTCP and inhibiting the phosphorylation of p38.The combined use of oxymatrine and entecavir could significantly increase anti-HBV effect.