固本健脑方调节健忘大鼠海马组织中AGEs/RAGE信号通路的探讨

姜瑞雪; 黄密; 马作峰; 李庆琳; 孙晓利

doi:10.13422/j.cnki.syfjx.2017010153

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固本健脑方调节健忘大鼠海马组织中AGEs/RAGE信号通路的探讨

药理 | 更新时间：2024-01-04

- 固本健脑方调节健忘大鼠海马组织中AGEs/RAGE信号通路的探讨
- Guben Jiannao Prescription Regulates AGEs/RAGE Signaling Pathway in Hippocampus of Forgetful Aged Rats
- 中国实验方剂学杂志 2017年23卷第1期页码：153-157
- 作者机构：
- 作者简介：
- 基金信息：
  
  湖北省卫生厅2012年度中医药中西医结合科研重点项目（2012Z-Z02）;湖北省自然科学基金项目（2013CFC109，2014CFB454）;湖北中医药大学大学生创新基金项目（2015）
- DOI：10.13422/j.cnki.syfjx.2017010153
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
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姜瑞雪, 黄密, 马作峰, 等. 固本健脑方调节健忘大鼠海马组织中AGEs/RAGE信号通路的探讨[J]. 中国实验方剂学杂志, 2017,23(1):153-157.

JIANG Rui-xue, HUANG Mi, MA Zuo-feng, et al. Guben Jiannao Prescription Regulates AGEs/RAGE Signaling Pathway in Hippocampus of Forgetful Aged Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(1): 153-157.
姜瑞雪, 黄密, 马作峰, 等. 固本健脑方调节健忘大鼠海马组织中AGEs/RAGE信号通路的探讨[J]. 中国实验方剂学杂志, 2017,23(1):153-157. DOI： 10.13422/j.cnki.syfjx.2017010153.

JIANG Rui-xue, HUANG Mi, MA Zuo-feng, et al. Guben Jiannao Prescription Regulates AGEs/RAGE Signaling Pathway in Hippocampus of Forgetful Aged Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(1): 153-157. DOI： 10.13422/j.cnki.syfjx.2017010153.

摘要

目的：基于晚期糖基化终末产物（AGEs）/高级糖基化终产物受体（RAGE）信号通路，探讨固本健脑方对老年健忘大鼠海马脑区神经炎性反应的调控作用。方法：腹腔注射D-半乳糖（120 mg·kg-1）及NaNO2（90 mg·kg-1）建立老年健忘大鼠模型，分别ig固本健脑方（16.7 g·kg-1），归脾丸（16.3 g·kg-1），六味地黄丸（12.5 g·kg-1）和石杉碱甲（100 μg·kg-1），并设正常组和模型组，ig等体积生理盐水。给药共20 d后，取海马组织，采用免疫组化法检测AGEs蛋白的表达，蛋白质免疫印迹（Western blot）检测RAGE及核转录因子-κB p65（NF-κB p65）蛋白的表达，酶联免疫吸附测定（ELISA）检测白细胞介素-1β（IL-1β），肿瘤坏死因子-α（TNF-α）的含量。结果：与正常组比较，模型组大鼠海马组织AGEs蛋白，RAGE及NF-κB p65蛋白的表达，IL-1β及TNF-α含量均明显上调（P < 0.05，P < 0.01）；与模型组比较，各给药组明显下调大鼠海马组织AGEs蛋白，RAGE及NF-κB p65蛋白的表达，IL-1β及TNF-α含量（P<0.01）；与固本健脑方组比较，其余给药组上述指标的表达上调（P<0.05）；归脾丸组、六味地黄丸组、石衫碱甲组组间比较，差异无统计学意义。结论：上述方药均能在一定程度上改善老年健忘大鼠的记忆功能，但固本健脑方作用最佳，其分子机制与AGEs/RAGE信号通路密切相关。

Abstract

Objective: To explore the regulating effect of Guben Jiannao prescription on neuro-inflammatory reaction in hippocampus of the forgetful aged rats based on advanced glycosylation and products(AGEs)/advanced glycosylation end products receptor(RAGE) signal pathway. Method: Intra-peritoneal injection of D-galactose and NaNO2was used to establish the forgetful models in aged rats. Then they were treated with Guben Jiannao prescription (16.7 g·kg-1)

Guipiwan (16.3 g·kg-1)

Liuwei Dihuangwan (12.5 g·kg-1) and huperzine A (100 μg·kg-1) respectively by ig administration. The rats in normal group and model group received the same volume of normal saline. After treatment for 20 days

hippopocamus tissues were taken from all groups. The protein expression of AGEs was detected by immunohistochemistry. RAGE and nuclear factor-κB p65(NF-κB p65) protein expression levels were detected by Western blot. The contents of interleukim-1β(IL-1β) and tumor necrosis factor-α(TNF-α) were detected by ELISA. Result: As compared with the normal group

the levels of all above indices were enhanced significantly in model group (P < 0.05

P < 0.01). As compared with the model group

the levels of all above indices were significantly decreased in various treatment groups (P<0.01). As compared with Guben Jiannao prescription

the levels of all above indices were up-regulated in other treatment groups (P<0.05); there was no statistical significance in the levels of all above indices between Guipi Wan group

Liuwei Dihuang Wan group and huperzine A group. Conclusion: All of the above Chinese herbal compound prescriptions can improve memory function of forgetful aged rats to a certain degree

but the effect of Guben Jiannao Prescription is best. Its molecular mechanism is closely related to AGEs/RAGE signaling pathway.

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