Objective: To investigate the protective effect and mechanism of Shenxiong Glucose injection (SGI) on H2O2-induced oxidative damage of HUVEC cells. Method: The in vitro models of oxidative stress damage based on HUVEC cells were established by treatment with H2O2(130 mmol·L-1) for 0.5 h. SGI pretreated group:HUVEC cells were pretreated with SGI (6%

8%

10%) for 6 h

and then treated with H2O2 for 0.5 h. The cell viability was detected by MTS assay; ELISA method was used to determine lactate dehydrogenase(LDH) release

malondialdehyde(MDA) content as well as superoxide dismutase(SOD)

catalase(CAT)

glutathione peroxidase(GSH-Px) activities; gene and protein expression levels of Bcl-2

Bax and Caspase-3 in HUVEC cells were detected by Real-time PCR and Western blot. Result: After HUVEC cells were treated with H2O2(130 mmol·L-1) for 0.5 h

the survival rate of the cells decreased to about 50%with a proper range and good repeatability

so that oxidative damage models were established under this condition. As compared with the H2O2 group

the cells survival was significantly increased (P < 0.05

P < 0.01) after treatment with SGI for 6 h. SGI not only decreased the release of LDH

MDA (P < 0.05

P < 0.01)

but also significantly increased the activities of SOD

GSH-Px

and CAT (P < 0.05

P < 0.01). In addition

Real-time PCR and Western blot showed SGI can significantly up-regulate the expression level of Bcl-2 (P < 0.05

P < 0.01) and down-regulate the expression level of Caspase-3

Bax (P < 0.05

P < 0.01). Conclusions: SGI could protect the HUVEC cells against H2O2-induced oxidative damage in a certain range. The mechanism of action seemed to be relevant with inhibiting apoptosis.