Objective: To establish a GC chromatographic fingerprint analysis method for the quality evaluation of Dieda Huoxue powder. Method: The fingerprint chromatography was performed by GC. The similarity was evaluated with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (version 2008). Common peaks were identified by GC-MS through the comparison with the references

literature

and/or NIST database. Meanwhile

their origins were determined through the comparison with reference drugs. Result: The precision

repeatability and stability of the established method can meet the requirements of fingerprint chromatography analysis. There were 20 common peaks in the GC fingerprint

among which 17 peaks were identified. The similarity of 14 batches of samples ranged from 0.564 to 0.986

indicating greater differences in the qualities among the samples. Conclusion: The developed method could provide the comprehensive information about the chemical components in Dieda Huoxue powder in a rapid and convenient manner. It could be used in quality evaluation of the drug.