醒脑益智方对T2DM痴呆小鼠性激素信号通路的影响

高晓斐; 徐萌; 张静; 岳仁宋

doi:10.13422/j.cnki.syfjx.2017020081

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醒脑益智方对T2DM痴呆小鼠性激素信号通路的影响

药理 | 更新时间：2024-01-04

- 醒脑益智方对T2DM痴呆小鼠性激素信号通路的影响
- Effect of Xingnao Yizhi Prescription on Sex Hormone Signaling in Diabetic Dementia Mice
- 中国实验方剂学杂志 2017年23卷第2期页码：81-85
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81072775）;四川省学术和技术带头人培养资金项目（CRS2014001）;四川省科技计划项目（2015SZ0100）
- DOI：10.13422/j.cnki.syfjx.2017020081
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
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高晓斐, 徐萌, 张静, 等. 醒脑益智方对T2DM痴呆小鼠性激素信号通路的影响[J]. 中国实验方剂学杂志, 2017,23(2):81-85.

GAO Xiao-fei, XU Meng, ZHANG Jing, et al. Effect of Xingnao Yizhi Prescription on Sex Hormone Signaling in Diabetic Dementia Mice[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(2): 81-85.
高晓斐, 徐萌, 张静, 等. 醒脑益智方对T2DM痴呆小鼠性激素信号通路的影响[J]. 中国实验方剂学杂志, 2017,23(2):81-85. DOI： 10.13422/j.cnki.syfjx.2017020081.

GAO Xiao-fei, XU Meng, ZHANG Jing, et al. Effect of Xingnao Yizhi Prescription on Sex Hormone Signaling in Diabetic Dementia Mice[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(2): 81-85. DOI： 10.13422/j.cnki.syfjx.2017020081.

摘要

目的：探讨醒脑益智方对2型糖尿病痴呆小鼠血清性激素水平及雌激素受体（estrogen receptor，Er）α，Erβ mRNA表达的影响及其作用机制。方法：设昆明小鼠为空白组，将KK-Ay小鼠血糖≥13.9 mmol·L-1者随机分为模型组、吡拉西坦组、醒脑益智方组。吡拉西坦组ig吡拉西坦片（150 mg·kg-1），醒脑益智方组ig醒脑益智方（7.2 g·kg-1），空白组和模型组每天用等体积生理盐水ig。给药前后行Morris水迷宫测试并记录潜伏期。小鼠麻醉后迅速取血、大脑海马及额叶皮质。酶联免疫吸附法（ELISA）测定血浆性激素水平，包括卵泡刺激素（FSH），孕酮（P），雌二醇（E2），睾酮（T）等；实时荧光定量PCR检测Erα，Erβ mRNA表达水平。结果：与模型组比较，空白组、吡拉西坦组和醒脑益智方组第5天潜伏期明显减少（P<0.05）；与模型组比较，空白组、醒脑益智方组促黄体生成素（LH），T水平明显下降（P<0.05），E2，P水平明显升高（P<0.05）；与吡拉西坦组比较，醒脑益智方组LH水平明显下降（P<0.05）。与模型组比较，吡拉西坦组、醒脑益智方组Erβ受体mRNA表达水平明显增高（P<0.05），其他各组间差异均不明显。结论：糖尿病痴呆的出现可能与性激素通路有关，醒脑益智方对糖尿病轻度认知功能障碍有明显改善作用，这一作用可能通过提高血浆E2，P水平及雌激素受体Eβ表达水平，降低LH，T水平实现。

Abstract

Objective: To investigate the effect and mechanism of Xingnao Yizhi prescription on mRNA expression levels of serum hormone and estrogen receptor(Erα and Erβ) for type 2 diabetic dementia mice. Method: Kunming mice were set as blank group. KK-AY mice with blood sugar ≥13.9 mmol·L-1 were randomly divided into model group

piracetam group

and Xingnao Yizhi prescription group. Piracetam tablets (150 mg·kg-1) were given by ig administration in piracetam group. Xingnao Yizhi prescription (7.2 g·kg-1) was given by ig administration in Xingnao Yizhi prescription group. Equal volume of saline was given in normal group and model group by ig administration. Morris water maze test was conducted before and after drug administration

and latent period was recorded. Plasma

hippocampus samples and frontal cortex were collected as soon as possible after anesthesia in mice. Enzyme linked immunosorbent assay (ELISA) was used to test plasma sex hormone level

such as follicle-stimulating hormone (FSH)

progesterone (P)

estradiol (E2)

and testost erone (T). Real-time PCR was used to test Erα

Erβ mRNA expression levels. Result: As compared with model group

the latent period was significantly shortened in blank group

piracetam group

and Xingnao Yizhi prescription group (P<0.05); LH and T levels were significantly reduced in blank group and Xingnao Yizhi prescription group (P<0.05)

and E2 and P levels were significantly increased (P<0.05). As compared with piracetam group

LH level was significantly reduced in Xingnao Yizhi prescription group (P<0.05). As compared with the model group

Erβ mRNA expression level was significantly increased in piracetam group and Xingnao Yizhi prescription group (P<0.05). Conclusion: The emergence of diabetic dementia may be related to the sex hormone pathway. Xingnao Yizhi prescription can improve the mild cognitive impairment in patients with diabetes mellitus

and the mechanism may be associated with improving plasma E2

P levels and Erβ mRNA expression levels

and reducing LH and T levels.

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