Objective: To investigate the anti-oxidation and anti-inflammatory effects of resveratrol (Res) on renal tissues in gestational diabetic rats. Method: Female rats were fed with high fat and high sugar diet for 8 weeks and then kept in the same cage with male rats to prepare pregnant rats. After 5 days

the pregnant rats were injected with STZ (35 mg·kg-1) by intraperitonal to make gestational diabetic models. 80 model rats were selected andrandomly devided into model group

Res (60

120

240 mg·kg-1) groups and Metformin hydrochloride (200 mg·kg-1) group. Another 16 gestational rats in the same batch were selected as normal gestational group

and 16 non-gestational female rats at the same age were selected as normal non-gestational group. After successful modeling

the medicines were given by intragastric administration for 2 weeks

once a day. Before and after treatment

the general status of rats in every group were observed. After treatment

the levels of blood sugar were determined; the level of 24 h urine protein (UPro) was determined; the levels of blood urea nitrogen (BUN)

creatinine (SCr)

and uric acid (UA) in serum were determined; the renal tissue histopathological changes were observed by periodic acid-schiff staining (PAS) method; the renal cells apoptosis was observed by terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) staining method

and the apoptosis index was alculated; the levels of total antioxidative capacity (T-AOC) in serum and the activities of superoxide dismutase (SOD)

glutathione peroxidase (GSH-Px)

catalase (CAT) as well as the content of malondialdehyde (MDA) in renal tissues were determined; the levels of C-reactionprotein (CRP)

tumor nerosis factor-α (TNF-α)

interlukin-1β (IL-1β)

interlukin-6 (IL-6)

and intercellular cell adhesion molecule-1 (ICAM-1) in plasma were also determined. Result: There were no differences in various monitoring indexes between the normal gestation group and normal non-gestation group. As compared with the normal gestation group

the blood sugar level was significantly increased in model group (P<0.01); the 24 h urine volume

UPro and the contents of BUN

SCr

UA in serum were significantly increased (P<0.01); with obvious histopathological changes in renal tissues and renal cells apoptosis

and the apoptosis index was significantly increased (P<0.01); the level of T-AOC and the activities of SOD

GSH-Px

CAT in renal tissues were significantly decreased

and the content of MDA in renal tissue was significantly increased (P<0.01). As compared with the model group

the general status of the rats in Res groups were improved; the symptoms were relieved; the levels of blood sugar in Res 120 and 240 mg·kg-1 groups were significantly decreased; the 24 h urine volume and the UPro level were significantly decreased

and the contents of BUN

SCr

UA in serum were significantly decreased; the histopathological changes in renal tissues and renal cells apoptosis were significantly improved

and the apoptosis indexes were significantly decreased; the activities of SOD

CAT in renal tissues were significantly increased

and the content of MDA was significantly decreased; the levels of T-AOC and the activity of GSH-Px in renal tissues of Res 240 mg·kg-1 group were significantly increased (P < 0.05

P < 0.01). Conclusion: Res had anti-oxidation and anti-inflammatory effects on renal tissues in diabetic rats.