Objective: To optimize extraction technology of couplet medicines of Sophorae Flavescentis Radix-Glycyrrhizae Radix et Rhizoma and to quickly identify the chemical constituents in its extract. Method: Taking transfer rates of matrine

oxymatrine and glycyrrhizic acid as comprehensive evaluation index

based on single factor tests

optimum extraction conditions of couplet medicines extract were selected by orthogonal test.The chemical constituents of couplet medicines extract were identified on-line by UPLC-Q-TOF/MS. Result: Optimum reflux extraction process was as follows:soaked for 1 h and extracted twice with 8 times the amount of 60% ethanol

2 h for each time.Total transfer rate of matrine and oxymatrine was 93.92%

the extraction yield of glycyrrhizic acid was 99.23%.Furthermore

a total of 49 constituents were identified in couplet medicines extract

28 constituents were from Sophorae Flavescentis Radix and 21 constituents were from Glycyrrhizae Radix et Rhizoma. Conclusion: The mixed extract has a certain auxo-action for extraction of matrine

oxymatrine and glycyrrhizic acid and the optimum extraction process is stable and feasible.Identification of chemical constituents in extract will be useful for elucidation of its active constituents and compatible mechanism.