Objective: To isolate and identify the contents of scutebarbatine A from Scutellariae Barbatae Herba. Method: Scutebarbatine A was isolated from Scutellariae Barbatae Herba by chromatographic separation method and its structure was determined by spectroscopic method. Agilent-Eclipse XDB-C18 column (4.6 mm×250 mm

5 mm) was used with methanol-water (60:40) as the mobile phase at a flow rate of 1 mL·min-1. The detection wavelength was set at 260 nm and the column temperature was 40℃. This method was used to investigate the differences of scute barbatine A contents in Scutellariae Barbatae Herba of different origins. Result: The calibration curves of scutebarbatine A showed good linearity over the ranges of 0.030 34-1.213 6 μg (r=0.999 9). The repeatability RSD was 1.8%; precision RSD was 0.5%; and the test sample solution was stable within 16 h

with an average recovery rate of 100.2% (RSD 3.6%). Conclusion: The quantitative determination method to isolate and identify scutebarbatine A from scutellariae Barbatae Herba is controllable

scientific

and suitable for the quality control of Scutellariae Barbatae Herba.