Objective: To study the chemical constituents of the anti-inflammatory fractions from the formula granules of Artemisiae Scopariae Herba. Method: The compounds were isolated and purified by recrystallization

silica gel

Sephadex LH-20

and semi-preparative HPLC. Their structures were identified on the basis of spectroscopic methods combined with physicochemical characteristics. Result: From the systemic analysis on the chemical constituents of the anti-inflammatory fractions from the formula granules of Artemisiae Scopariae Herba (macroporous resin 50%ethanol

and 100%ethanol)

thirteen compounds were isolated from the anti-inflammatory fractions and were identified as 4'-hydroxyacetophenone (1)

2

5-dihydroxycinnamic acid ethyl ester (2)

quercetin (3)

luteolin (4)

7S

8R-dihydrodehydroconiferyl alcohol (5)

prudomestin (6)

7

4'-dimethoxykaempferol (7)

quercetin-7-O-α-L-rhamnoside (8)

9S

12S

13S-trihydroxy-10E-octadecenoate (9)

isorhmnetin-4'-O-β-D-glucoside (10)

kaempferol-3-O-β-D-glucoside (11)

stearic acid (12) and β-daucosterol (13) respectively. Conclusion: All the above 13 compounds were obtained from the formula granules of Artemisiae Scopariae Herba for the first time; compounds 9-11 were obtained from genus Artemisia for the first time; and compounds 2 and 5 were isolated from Artemisia scoparia for the first time.