延胡索中3种成分近红外同步定量检测模型的建立

顾超; 岳显可; 杜伟锋; 石森林; 葛卫红

doi:10.13422/j.cnki.syfjx.2017030060

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延胡索中3种成分近红外同步定量检测模型的建立

化学与分析 | 更新时间：2024-01-04

- 延胡索中3种成分近红外同步定量检测模型的建立
- Establishment of NIRS Quantitative Model for Synchronous Quantitative Detection of Contents of Three Alkaloids in Corydalis Rhizoma
- 中国实验方剂学杂志 2017年23卷第3期页码：60-65
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家中医药行业专项（201507002）;浙江省重中之重一级学科—中药学开放基金项目
- DOI：10.13422/j.cnki.syfjx.2017030060
  中图分类号： R284.1
- 纸质出版日期：2017
- 稿件说明：
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顾超, 岳显可, 杜伟锋, 等. 延胡索中3种成分近红外同步定量检测模型的建立[J]. 中国实验方剂学杂志, 2017,23(3):60-65.

GU Chao, YUE Xian-ke, DU Wei-feng, et al. Establishment of NIRS Quantitative Model for Synchronous Quantitative Detection of Contents of Three Alkaloids in Corydalis Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(3): 60-65.
顾超, 岳显可, 杜伟锋, 等. 延胡索中3种成分近红外同步定量检测模型的建立[J]. 中国实验方剂学杂志, 2017,23(3):60-65. DOI： 10.13422/j.cnki.syfjx.2017030060.

GU Chao, YUE Xian-ke, DU Wei-feng, et al. Establishment of NIRS Quantitative Model for Synchronous Quantitative Detection of Contents of Three Alkaloids in Corydalis Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(3): 60-65. DOI： 10.13422/j.cnki.syfjx.2017030060.

摘要

目的：建立延胡索近红外定量检测模型，快速测定延胡索中去氢紫堇碱、延胡索乙素、紫堇碱的含量。方法：通过HPLC测定延胡索中去氢紫堇碱、延胡索乙素、紫堇碱的化学值，并通过近红外光谱技术分别采集其近红外光谱，将化学值与近红外光谱一一对应，以偏最小二乘法建立校正模型，采用一阶求导和多元散射校正对模型进行优化，形成最佳延胡索近红外定量检测模型。结果：建立的去氢紫堇碱、延胡索乙素、紫堇碱校正模型的相关系数分别为0.987，0.998，0.997。通过验证集验证，去氢紫堇碱、延胡索乙素、紫堇碱3种生物碱成分真实值与预测值的相关系数分别为0.943，0.978，0.991，相对偏差分别为-14.75%~18.08%，-4.73%~9.23%，-10.99%~9.70%。结论：建立的近红外定量模型预测精度较高，可以用于延胡索3种生物碱成分的快速同步测定。

Abstract

Objective: To establish the near-infrared spectroscopy(NIRS) quantitative model to determine the contents of dehydrocorydaline

tetrahydropalmatine and corydaline in Corydalis Rhizoma rapidly. Method: High performance liquid chromatography (HPLC) was used to determine the authentic contents of dehydrocorydaline

tetrahydropalmatine and corydaline in Corydalis Rhizoma.The spectrum was collected through near infrared diffuse reflection spectroscopy. Then the authentic contents were corresponded to the near infrared spectrum one by one. The correction model was established by partial least squares(PLS) and optimized by multiple scattering correction (MSC) & first derivative. Result: Correlation coefficient (R) was 0.987

0.998

and 0.997 respectively fordehydrocorydaline

tetrahydropalmatine

and corydalinecorrection models respectively. The validation set indicated that the correlation coefficient between predicted content and authentic content of dehydrocorydaline

tetrahydropalmatine and corydaline was 0.943

0.978 and 0.991 respectively

and their relative deviation was -14.75%-18.08%

-4.73%-9.23% and -10.99%-9.70% respectively. Conclusion: The established NIRS quantitative model had high prediction accuracy

and could be used to determine the contents of three alkaloids in Corydalis Rhizoma rapidly and synchronously.

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