Objective: To screen the diuretic active fractions from rattan of Akebia trifoliate and study its chemical constituents. Method: The 95%ethanol extract of rattan of A. trifoliate

which were extracted with petroleum ether

ethyl acetate

butanol and water dissolving fractions

were investigated through metabolic cages experiments. The compounds of active fractions were isolated and purified by silica gel column chromatography and other methods

and their structures were identified by NMR and MS combined with literature data. Result: The butanol extract fraction can significantly increase the urine volume of rats(P<0.01)

but the diuretic effect of other fractions was not obvious. Nine compounds were isolated from this diuresis fraction

and their structures were identified as D-galactose (1)

syringicacid-4-O-α-L-rhamnopyranoside (2)

hederagenin(3)

inositol(4)

mannitol (5)

vanillic acid-β-glucoside (6)

glucosyringic acid (7)

hederagenin-3-O-α-L-arabinopyranoside (8) and β-sitosterol-3-O-β-D-glycoside (9). Conclusion: The butanol extract fraction had significant diuretic function

and glycosides were the main chemical composition of this fraction.