Objective: To establish the method for extraction

isolation and determination of ellagic acid and 3

3-di-O-methylellagic acid from Macrosolen cochinchinensis. Method: The ellagic acid and 3

3-di-O-methylellagic acid were isolated by solvent extraction

silica gel column chromatography and recrystallization techniques. Then HPLC content determination method was established:Eclipse XDB-C18 chromatographic column (4.6 mm×250 mm

5 μm) was used with methanol-0.2% phosphoric acid (47:53) as the mobile phase; the flow rate was 0.8 mL·min-1; UV detection wavelength was set at 254 nm and the column temperature was 25℃. Result: Ellagic acid showed good linear relationship within 0.030 6-0.551 2 μg (r=0.999 8) and its average recovery rate was 101.25%

RSD 0.9%. 3

3-di-O-methylellagic acid showed good linear relationship within 0.018 9-0.339 5 μg (r=0.999 8) and its average recovery rate was 101.34%

RSD 1.5% (n=6). Conclusion: The ellagic acid and 3

3-di-O-methylellagic acid were obtained from M. cochinchinensis for the first time

and the HPLC method was established for content determination. This method was simple

rapid and with great stability and repeatability

which could be used to determine the contents of above two components from M. cochinchinensis.