Objective: To explore the protective effect and mechanism of active components compatibility of ethyl acetate extracts(HE) and fatty oil(HSO) of Herpetospermum caudigerum on immunologic liver injury induced by concanavalin A (ConA) in mice. Method: The 80 mice were randomly divided into 8 groups (n=10)

normal group

model group

biphenyl double ester group (positive control)

HSO group

HE group

HSO and HE of 1:1

1:2

2:1 ratio groups. After ig administration of ethyl acetate extracts

fatty oil and different compatibilities for 8 days in treatment group

the mice were injected with ConA (30 mg·kg-1) through tail vein to establish the model of immunological liver injury. Blood was collected after 8 hours modeling to prepare liver homogenate. Liver and spleen indexes were calculated; the contents of alanine aminotransferase (ALT)

aspartate aminotransferase (AST) and nitrogen monoxidum (NO) in serum

superoxide dismutase (SOD)

malondialdehyde (MDA) in liver homogenate were assayed by ELISA; and pathological morphological changes in liver cells were monitored by HE. Result: Ethyl acetate extracts

fatty oil and their combinations of H. caudigerum could decrease liver and indexes

the levels of ALT

AST

NO

MDA and increased SOD activities. Meanwhile

the pathological damage in liver was relieved. As compared with the model group

thefatty oiland ethyl acetate extracts at 2:1 ratio group showed the best effect on immunological liver injury in mice

with significant differences in above indexes. Conclusion: Ethyl acetate extracts compatibility with oil can protect immunological liver injury in mice

and the effect was superior to that of single component. The mechanism may be associated with anti-inflammatory

anti-oxidation and scavenging oxygen free radical.