Objective: To optimize supercritical CO2 fluid extraction process of volatile oil from Angelicae Sinensis Radix and provide reference for its further research and development. Method: UPLC was adopted to determine the contents of coniferyl ferulate and ligustilide in volatile oil from Angelicae Sinensis Radix with mobile phase of acetonitrile-water (50:50)

detection wavelength at 320 nm and the flow rate of 0.4 mL·min-1.With extracting amounts of coniferyl ferulate and ligustilide as indexes

extraction parameters including temperature

pressure and time were optimized by orthogonal test. Result: Coniferyl ferulate and ligustilide showed good linearity at 0.006 2-0.777 6 μg and 0.017 9-2.238 μg

respectively;their recoveries were between 96.67%-102.08%.Optimum extraction parameters were as follows:extraction temperature at 40℃

extraction pressure of 25 MPa and extraction time of 2 h;average extracting amounts of coniferyl ferulate and ligustilide were 1.62

10.28 mg·g-1

respectively. Conclusion: This UPLC is repeatable and simple

it can be used for quality control of volatile oil from Angelicae Sinensis Radix.Optimum extraction process is stable and feasible for extraction of volatile oil from Angelicae Sinensis Radix.