Objective: To study the chemical constituents of the ethyl acetate extract of Lonicerae Japonicae Caulis made in Shandong. Method: Lonicerae Japonicae Caulis made in Shandong was crushed into coarse powder

extracted by 70% ethanol

concentrated under reduced pressure

and then its ethyl acetate extract was gained by fractional extraction. Chemical compounds were isolated and purified by using repeated column chromatographies such as silica gel

MCI-gel resin

and Sephadex LH-20.Their structures were elucidated by spectral data and physicochemical properties. Result: Ten compounds were obtained and identified as syringic acid (1)

ursolic acid (2)

pinoresinol (3)

oleanolic acid (4)

diosmetin (5)

β-sitosterol (6)

cinnamic acid (7)

apigenin (8)

luteolin (9)

and quercetin (10). Conclusion: Compounds 1-3 were isolated from Lonicera for the first time

and compounds 4-5 were isolated from Lonicerae Japonicae Caulis for the first time.