Objective: To establish an HPLC-fingerprint (HPLC-FP) analysis method for Elaeagnus pungens leaves and provide a new method for their quality control. Method: HPLC separation was performed on Agilent ZOTBAX XDB-Phenyl C18(4.6 mm×250 mm

5 μm) with 0.1% phosphoric acid-acetonitrile as the mobile phase for gradient elution; the column temperature was 30℃; the flow rate was 1.0 mL·min-1; the detection wavelength was set at 360 nm. The Similarity Evaluation System for Chromatographic Fingerprint of TCM (2004A edition) was used to evaluate the similarity of 12 batches of E. pungens leaves. Result: With tiliroside as the reference peak

sixteen common peaks were obtained in 12 batches of samples. The similarity of each fingerprint within one group was over 0.80

and squared Euclidean distance method was used for clustering analysis. Conclusion: This method shows high precision

stability and good reproducibility

and it can provide basis for the quality control of E. pungens leaves.