Objective: According to urine metabonomics differences between spontaneously hypertensive rats (SHR) and WKY rats

to investigate effect of Danqi tablets on blood pressure and urine metabolomics of SHR. Method: SHR and WKY rats were used in the study.SHR were randomly divided into Danqi tablets group(dose of 0.38 g·kg-1)

aspirin group (10 mg·kg-1)

Danqi tablets+aspirin group (dose of 0.38 g·kg-1 and 10 mg·kg-1).Systolic blood pressure (SBP) of rat tail artery was constantly monitored before and after administration and rat urine in 24 hours was collected.All urine samples were detected by 1H-NMR.Partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were used to discussed effect of Danqi tablets and aspirin on metabolites in urine of SHR. Result: Compared with the model group

blood pressure of all groups was significantly lower after 4 weeks.Compared with WKY rats

21 kinds of different metabolites were found in SHR urine

they mainly included alanine

succinate

dimethylamine and so on.Danqi tablets

aspirin and Danqi tablets+aspirin all can make the distribution of a part of different metabolites in SHR urine back to normal. Conclusion: Danqi tablets and Danqi tablets+aspirin can lower the blood pressure of SHR

these two kinds of drugs can adjust distribution of relevant differetial metabolites associated with hypertension.