哈巴俄苷在大鼠胃肠道内容物中的稳定性及其入血成分分析

刘文君; 孟兆青; 孙林; 程宁波; 黄文哲; 王振中; 王跃生; 萧伟

doi:10.13422/j.cnki.syfjx.2017040108

您当前的位置：

首页 >

文章列表页 >

哈巴俄苷在大鼠胃肠道内容物中的稳定性及其入血成分分析

药物代谢 | 更新时间：2024-01-04

- 哈巴俄苷在大鼠胃肠道内容物中的稳定性及其入血成分分析
- Stability of Harpagoside in Gastrointestinal Contents of Rats and Analysis of Its Constituents Absorbed in Rat Serum After Oral Administration
- 中国实验方剂学杂志 2017年23卷第4期页码：108-111
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家“重大新药创制”科技重大专项（2013ZX09402203）
- DOI：10.13422/j.cnki.syfjx.2017040108
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
移动端阅览
刘文君, 孟兆青, 孙林, 等. 哈巴俄苷在大鼠胃肠道内容物中的稳定性及其入血成分分析[J]. 中国实验方剂学杂志, 2017,23(4):108-111.

LIU Wen-jun, MENG Zhao-qing, SUN Lin, et al. Stability of Harpagoside in Gastrointestinal Contents of Rats and Analysis of Its Constituents Absorbed in Rat Serum After Oral Administration[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(4): 108-111.
刘文君, 孟兆青, 孙林, 等. 哈巴俄苷在大鼠胃肠道内容物中的稳定性及其入血成分分析[J]. 中国实验方剂学杂志, 2017,23(4):108-111. DOI： 10.13422/j.cnki.syfjx.2017040108.

LIU Wen-jun, MENG Zhao-qing, SUN Lin, et al. Stability of Harpagoside in Gastrointestinal Contents of Rats and Analysis of Its Constituents Absorbed in Rat Serum After Oral Administration[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(4): 108-111. DOI： 10.13422/j.cnki.syfjx.2017040108.

摘要

目的：研究哈巴俄苷在生物样品中的稳定性及其大鼠灌胃后的入血成分。方法：利用HPLC测定哈巴俄苷的含量，流动相0.03%磷酸水溶液-乙腈梯度洗脱，检测波长210 nm，计算其在各样品中的剩余率，研究哈巴俄苷在人工胃液、人工肠液、大鼠胃肠内容物及相应pH溶液中的稳定性；利用LC-MS检测大鼠灌胃给药后的血液样品，对哈巴俄苷的入血成分进行定性研究。结果：哈巴俄苷在人工肠液、大鼠小肠内容物、大鼠大肠内容物中较稳定，降解率<10%；在稀酸液、人工胃液、大鼠胃内容物中哈巴俄苷可发生降解，但降解率<15%；大鼠灌胃给予哈巴俄苷后的血液样品中检测到了哈巴俄苷、哈巴苷、肉桂酸。结论：哈巴俄苷在人工胃肠液、大鼠胃肠道内容物及相应pH溶液中较稳定，少部分在酸性环境可分解成肉桂酸及哈巴苷苷元的脱水产物；哈巴俄苷大鼠灌胃给药后主要以原型入血，在体内可代谢为肉桂酸及哈巴苷。

Abstract

Objective: To investigate stability of harpagoside in biological fluids in vitro and analyze its constituents absorbed in rat serum after oral administration. Method: Harpagoside was dissolved in artificial gastric juice

intestinal juice and gastrointestinal contents of rats

the corresponding pH solution

respectively;then thermostatically maintained at 37℃.HPLC was used to calculate the remaining rate of harpagoside in biological samples by measuring the content of harpagoside at time intervals after degradation.Constituents absorbed in rat serum were detected by LC-MS after oral administration. Result: Harpagoside was relatively stable in artificial intestinal juice and intestinal contents of rats

its degradation rates were less than 10%;however

it could be degraded in diluted acid

artificial gastric juice and gastric contents of rats

its degradation rates were less than 15%.Harpagoside

cinnamic acid and harpagide were detected in rat serum after oral administration of harpagoside. Conclusion: Harpagoside is relatively stable in artificial gastric juice

intestinal juice as well as gastrointestinal contents of rats

and a few of it in the acidic environment can be broken into cinnamic acid and dehydration product of harpagide aglycone.Harpagoside is mainly absorbed by prototype in rat serum after oral administration

and it can be metabolized to cinnamic acid and harpagide in vivo.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

HPLC测定增液承气口服液中哈巴苷和哈巴俄苷含量

中药自组装纳米策略在肿瘤治疗中应用的研究进展

芦西丁在不同种属肝微粒体和肝S9中Ⅰ相代谢及Ⅱ相磺酸化代谢的稳定性考察

经典名方中玄参的本草考证

岩藻甾醇标准样品的研制