Objective: To develop an HPLC method for quantitative determination of chlorogenic acid

scopoletin

resveratrol and morin in Mori Cortex.Method: An Eclipse-C18 column (4.6 mm×250 mm

5 μm) was adopted with acetonitrile-0.01% phosphoric acid as the mobile phase for gradient elution. The flow rate was 1.0 mL·min-1. The detection wavelength was set at 285 nm and the column temperature was 30℃.Result: The calibration curves showed good linear relationship in the ranges of 6.4-76.8 mg·L-1 (r=0.999 9) for chlorogenic acid

5.15-61.8 mg·L-1(r=0.999 6) for scopoletin

1.11-13.32 mg·L-1 (r=0.999 6) for resveratrol and 2.76-33.12 mg·L-1(r=0.999 7) for morin. The average recoveries were between 98.4% and 99.4%(RSD≤2%). The sample solution was steady within 12 h

and this method showed good repeatability. Through the determination of 5 batches of Mori Cortex samples

results showed that the contents of chlorogenic acid

scopoletin and morin were higher in Henan samples (batch number 140401); and the content of resveratrol was higher in Hubei samples (batch number D5090202).Conclusion: The method is precise and accurate for determination of four ingredients in Mori Cortex.