Objective: To develop a method for quantitative analysis of multiple-components by single marker (QAMS) for simultaneous determination of lovastatin and lovastatin acid in Fermentum Rubrum and its preparations.Method: Lovastatin was used as the internal reference substance

and the relative correction factor (RCF) of lovastatin acid was determined by RP-HPLC. Then the QAMS method was established for simultaneous determination of the two components. The durability of the established method was verified with varying HPLC instrument and chromatographic columns. Then the accuracy of the QAMS method was evaluated by comparing the contents of lovastatin acid with those obtained by the external standard method.Result: The RCF value showed good durability for both lovastatin and lovastatin acid. There were no significant differences between the quantitative results of QAMS method and external standard method.Conclusion: An accurate

rapid and highly durable QAMS method has been developed for simultaneous determination the contents and locations of lovastatin and lovastatin acid

so as to provide reliable basis for the application of QAMS method in quality control of Fermentum Rubrum and its preparations.