加味泽泻汤对NAFLD大鼠肝脏炎症信号通路相关蛋白表达的影响

郭雨雅; 孙晓琦; 方晶; 方南元; 薛博瑜

doi:10.13422/j.cnki.syfjx.2017050090

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加味泽泻汤对NAFLD大鼠肝脏炎症信号通路相关蛋白表达的影响

药理 | 更新时间：2024-01-04

- 加味泽泻汤对NAFLD大鼠肝脏炎症信号通路相关蛋白表达的影响
- Effect of Modified Zexietang on Expression of Proteins Related to Inflammatory Pathway in NAFLD Rat
- 中国实验方剂学杂志 2017年23卷第5期页码：90-94
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金青年项目（81403376）;江苏省自然科学基金青年项目（BK20130955）
- DOI：10.13422/j.cnki.syfjx.2017050090
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
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郭雨雅, 孙晓琦, 方晶, 等. 加味泽泻汤对NAFLD大鼠肝脏炎症信号通路相关蛋白表达的影响[J]. 中国实验方剂学杂志, 2017,23(5):90-94.

GUO Yu-ya, SUN Xiao-qi, FANG Jing, et al. Effect of Modified Zexietang on Expression of Proteins Related to Inflammatory Pathway in NAFLD Rat[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(5): 90-94.
郭雨雅, 孙晓琦, 方晶, 等. 加味泽泻汤对NAFLD大鼠肝脏炎症信号通路相关蛋白表达的影响[J]. 中国实验方剂学杂志, 2017,23(5):90-94. DOI： 10.13422/j.cnki.syfjx.2017050090.

GUO Yu-ya, SUN Xiao-qi, FANG Jing, et al. Effect of Modified Zexietang on Expression of Proteins Related to Inflammatory Pathway in NAFLD Rat[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(5): 90-94. DOI： 10.13422/j.cnki.syfjx.2017050090.

摘要

目的：探讨加味泽泻汤对高脂饮食诱导的大鼠非酒精性脂肪肝（NAFLD）模型的治疗机制。方法：SPF级大鼠分为空白组、模型组、加味泽泻汤组、多烯磷脂酰胆碱组，大鼠分别以10%或45%高脂纯化饲料喂养12周构建NAFLD模型。加味泽泻汤及多烯磷脂酰胆碱治疗组分别给药4周，空白组及模型组则给予相应体积双蒸水4周。以蛋白免疫印迹（Western blot）法检测Toll样受体4（TLR4），髓样分化因子88（MyD88），核转录因子-κB p65（NF-κB p65），p38丝裂原活化蛋白酶（p38 MAPK），磷酸化的p38丝裂原活化蛋白酶（p-p38），c-Jun氨基末端激酶（JNK），半胱氨酸蛋白水解酶-1（Caspase-1）在大鼠肝脏中的表达。结果：与空白组比较，模型组TLR4，MyD88，NF-κB p65，p-p65，p38 MAPK，p-p38，JNK及Caspase-1蛋白表达明显增高（P<0.05）；而加味泽泻汤组相应蛋白的表达量较模型组均显著下降（P<0.01）。结论：在高脂饮食诱导的大鼠NAFLD模型中，加味泽泻汤通过抑制TLR4/NF-κB，MAPK信号通路相关蛋白的表达，从而抑制肝脏炎症的产生、缓解肝脏的损伤，达到治疗NAFLD的目的。

Abstract

Objective: To explore the effects of modified Zexietang on treatment of non-alcoholic fatty liver disease (NAFLD) in rats.Method: NAFLD rat model was established by high fat feeding (10% or 45% high fat feed) for 12 weeks and divided into 4 groups:control group

model group

modified Zexietang group and polyene phosphatidyl choline group. After the administration for four weeks

the corresponding volume of double stilled water was given for four weeks. Western blot was used to detect the expression of Toll like receptor 4 (TLR4)

myeloid differentiation factor 88 (MyD88)

nuclear factor-kappaB p65 (NF-κB p65)

phosphorylated p65 (p-p65)

p38 mitogen activated protein kinase (p38 MAPK)

p-p38 (phosphorylated p38)

c-Jun-NH2-terminal kinase (JNK) and cysteinyl aspartate-specificproteases-1 (Caspase-1) in liver.Result: Compared with control group

the above proteins in model group were significantly increased (P<0.05); and proteins in the modified Zexietang group were significantly decreased compared with model group (P<0.01).Conclusion: Modified Zexietang could inhibit liver inflammation and alleviate liver damage. Its mechanism may be related to the inhibition of the expression of protein in TLR4/NF-κB and MAPK signal pathways.

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