Objective: To study the effect of compound Shihu mixture(CSM) single-dose administration on insulin signaling in db/db mice.Method: According of the fasting blood glucose and body weight

30 male db/db mice of 14-16 weeks were randomly divided into model group

Chinese medicine group 1 and Chinese medicine group 2; another 10 db/m mice were selected as normal group. After single-dose administration of CSM1 and CSM2

glucose tolerance(OGTT) levels were determined from the overall aspect. The changes in levels of 6-phosphofructokinase-1 (PFK)

pyruvate dehydrogenase (PDH)

and expression levels of insulin receptor (InsR) and Akt phosphorylation were determined at 30 min and 60 min of administration.Result: As compared with the normal group

the blood glucose OGTT levels were significantly increased in model group at 0

30

60

120 min; activities of PFK and PDH in hepatic cells were significantly decreased; the protein expression levels of InsR and Akt were significantly reduced at 30 min and 60 min (P<0.01). As compared with the model group

the single-dose administration of both CSM1 and CSM2 could significantly reduce blood glucose OGTT levels at 60

120 min (P<0.05

P<0.01); increase the activities of PFK and PDH in hepatic cells

and promote the phosphorylation levels of InsR Tyr1150/1151

Akt Ser473 and Thr308 in a time-dependent manner(P<0.05

P<0.01).Conclusion: Single-dose administration of both CSM1 and CSM2 could improve the glucose tolerance in db/db mice and increase the activities of PFK and PDH in hepatic cells. The mechanism may be associated with improving insulin signal transduction and promoting the phosphorylation of InsR Tyr150/1151

Akt Ser473 and Thr308.