Objective: To investigate the inhibitory effect of atractylenolide Ⅱ on Lovo cells and its mechanism.Method: Lovo cells were cultured in vitro

and treated with respectively atractylenolide Ⅱ at different concentrations to incubate for 24 h. The proliferation ability of Lovo cells was detected by 3-(4

5-dimethyl-2-thiazolyl)-2

5-diphenyl-2-H-tetrazolium bromide (MTT) assay. The apoptosis of Lovo cells impacted by atractylenolide Ⅱ were measured by flow cytometry (FCM)

and the effect of atractylenolide Ⅱ on the expression of poly ADP-ribose polymerase1 (PARP1) and cysteinyl aspartate specific proteinase-3 (Caspase-3) were detected by the Western blot.Result: Compared with the blank control group

at the dose of 150 mg·L-1

atractylenolide Ⅱ significantly inhibited the proliferation of Lovo cells (P<0.05). With the increase of the concentration of atractylenolide Ⅱ

cell survival rate was gradually decreasing. At the concentration of more than 300 mg·L-1

cell survival rate dropped to less than half. High-dose (300 mg·L-1) atractylenolide Ⅱ can significantly promote the apoptosis of Lovo cells (P<0.01). At the dose of 100 mg·L-1

atractylenolide Ⅱ can cause shear in PARP1 and Caspase-3. With the increase of drug concentration

the shearing effect was significantly enhanced. Atractylenolide Ⅱ can inhibit the growth and proliferation of Lovo cells

regulate the expression of PARP1 and Caspase-3

and then promote the apoptosis of cells.Conclusion: Atractylenolide Ⅱ can significantly inhibit the proliferation of Lovo cells and induce their apoptosis

its molecular mechanism may be related to the regulation of the expression of PARP1 and Caspase-3.