Objective: To investigate urine-reducing components in petroleum ether part of salt-processed Alpiniae Oxyphyllae Fructus and verify their pharmacodynamics for clarifying its material basis and mechanism of urine-reducing. Method: HPLC and other chromatographic means were employed to separate and purify medicinal compositions

the structures of these compounds were identified by physicochemical properties and 1H-NMR

13 C-NMR spectral data.Guinea pig bladder detrusor was prepared by isolated tracheal thermostatic perfusion experimental method and isolated in the Kerbs solution.The tension of 7-epi-teucrenone B on the isolated guinea pig bladder detrusor and the effect of 7-epi-teucrenone B on guinea pig bladder detrusor contraction incubation by four antispasmodic agents including acetylcholine

histamine phosphate

BaCl2 and dopamine were measured by BL-420S biological function experiment system. Result: Nine compounds were isolated and identified from petroleum ether part of salt-processed Alpiniae Oxyphyllae Fructus

such as nootkatone

11-hydroxy-valenc-1(10)-en-2-one

teucrenone

7-epi-teucrenone B

12-hydroxynootkatone and so on.By confirmation of pharmacodynamics

7-epi-teucrenone B was closely related to urine-reducing. Conclusion: 7-Epi-teucrenone B inhibited contractile tension of isolated bladder detrusor may be through the antagonizing release of H1 receptor of histamine phosphate

it may be the urine-reducing component in petroleum ether part of salt-processed Alpiniae Oxyphyllae Fructus fruits.