胆南星质量评价方法初探

赵启苗; 单国顺; 陈江宁; 高慧; 贾天柱

doi:10.13422/j.cnki.syfjx.2017060028

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胆南星质量评价方法初探

药剂与炮制 | 更新时间：2024-01-04

- 胆南星质量评价方法初探
- Preliminary Study on Quality Evaluation of Arisaema Cum Bile
- 中国实验方剂学杂志 2017年23卷第6期页码：28-31
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家中医药管理局行业专项（201507009-03）
- DOI：10.13422/j.cnki.syfjx.2017060028
  中图分类号： R284.1
- 纸质出版日期：2017
- 稿件说明：
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赵启苗, 单国顺, 陈江宁, 等. 胆南星质量评价方法初探[J]. 中国实验方剂学杂志, 2017,23(6):28-31.

ZHAO Qi-miao, SHAN Guo-shun, CHEN Jiang-ning, et al. Preliminary Study on Quality Evaluation of Arisaema Cum Bile[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 28-31.
赵启苗, 单国顺, 陈江宁, 等. 胆南星质量评价方法初探[J]. 中国实验方剂学杂志, 2017,23(6):28-31. DOI： 10.13422/j.cnki.syfjx.2017060028.

ZHAO Qi-miao, SHAN Guo-shun, CHEN Jiang-ning, et al. Preliminary Study on Quality Evaluation of Arisaema Cum Bile[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 28-31. DOI： 10.13422/j.cnki.syfjx.2017060028.

摘要

目的：建立胆南星中总胆酸、猪去氧胆酸及鹅去氧胆酸的含量测定方法，为该药物的质量评价提供检测手段。方法：采用紫外分光光度法，以鹅去氧胆酸为指标成分，建立胆南星中总胆酸的含量测定方法，检测波长379 nm。采用高效液相色谱-电雾式检测器法（HPLC-CAD），建立胆南星中猪去氧胆酸和鹅去氧胆酸的含量测定方法，流动相0.1%冰乙酸（A）-乙腈（B）梯度洗脱（0~20 min，40%~45% B；20~45 min，45%~55% B），流速0.8 mL·min-1，柱温30℃。结果：鹅去氧胆酸在0~2.435 g·L-1与吸光度呈良好线性关系，平均加样回收率98.98%，RSD 1.0%。猪去氧胆酸和鹅去氧胆酸分别在4.7~94，5.44~108.8 mg·L-1与峰面积呈良好线性关系，平均加样回收率依次为99.53%（RSD 1.0%）和99.58%（RSD 1.4%）。结论：建立的含量测定方法操作简便、准确度高、重复性好，适用于胆南星的质量评价。

Abstract

Objective: To establish a determination method of total cholic acid

hyodeoxycholic acid and chenodeoxycholic acid in Arisaema Cum Bile

which could provide an valuable mean to control its quality. Method: The analysis was performed on ultraviolet spectrophotometry to determine the content of total cholic acid in Arisaema Cum Bile with chenodeoxycholic acid as marker substance.HPLC-CAD was used to analyze determination of hyodeoxycholic acid and chenodeoxycholic acid in Arisaema Cum Bile. Result: In the range of 0-2.435 g·L-1

it showed good linear relationships between the concentration of chenodeoxycholic acid and absorbance.The average recovery was 98.98% with RSD of 1.0%.Hyodeoxycholic acid and chenodeoxycholic acid were linear with the peak area in the range of 4.7-94

5.44-108.8 mg·L-1

the average recovery was 99.53% and 99.58%

respectively. Conclusion: This method is simple

accurate and suitable for the quality evaluation of Arisaema Cum Bile.

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