Discrimination of Aromi Fructus from Different Origins Using Electronic Nose Combined with Headspace-Gas Chromatography-Mass Spectrometry Coupled and Chemometrics
LIU Meng-chu, ZOU Xiao-hong, LAN Lun-li, et al. Discrimination of Aromi Fructus from Different Origins Using Electronic Nose Combined with Headspace-Gas Chromatography-Mass Spectrometry Coupled and Chemometrics[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 35-42.
LIU Meng-chu, ZOU Xiao-hong, LAN Lun-li, et al. Discrimination of Aromi Fructus from Different Origins Using Electronic Nose Combined with Headspace-Gas Chromatography-Mass Spectrometry Coupled and Chemometrics[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 35-42. DOI: 10.13422/j.cnki.syfjx.2017060035.
Objective: To detect aroma characteristics of Aromi Fructus from different originsby using electronic nose combined with automatic headspace-gas chromatography-mass spectrometry (HS-GC-MS)in two aspects
namely overall aroma profile and specific aroma composition
in order to study material basis of Aromi Fructus's odors. Method: With Aromi Fructus of different varieties and from different origins-Guangdong
Yunnan
Burma
Vietnam and Laos as a research subject
the volatile components were detected by using electronic nose and HS-GC-MS technique.A bioplot was used to analyze the correlation among samples
E-nose gas sensors and chemical volatile compounds. Result: E-nose showed a good differentiation of Aromi Fructus from different origins
with the identification index of 83.9%.Homebred Amomum villosum was classified in class A
and imported Aromi Fructus was classified in class A.A total of 70 volatile components were identified by HS-GC-MS
including terpenes
esters
alcohols
ketone and alkane.Aromi Fructus of different varieties and from different origins have different composition and content
with the same classification with the results of E-nose.A bioplot of PLS displayed the high correlation between sensors s14
s8/s6/s3 and terpenes
esters and alcohols indicating that special sensors were highly correlated with some aroma constituents. Conclusion: Advanced chemometrics and E-nose are used to discriminate Aromi Fructus from different origins.By combining with HS-GC-MS technique
this study provides further experimental evidence for material bases of Aromi Fructus's odors.