Objective: To analyze the genetic relationship and quality differences among different regional Chrysanthemi Flos by evaluating the quality of germplasm resources of Huai Chrysanthemi Flos. Method: The methods of field observation

visiting herbalist were adopted to investigate and analyze the germplasm resources of Huai Chrysanthemi Flos. The contents of chlorogenic acid

luteoloside and 3

5-O-double coffee acyl quinic acid were determined by the method in the Chinese Pharmacopoeia of 2015. The content of flavonoids was determined by ultraviolet-visible spectrophotometry. The contents of moisture

total ash

acid insoluble ash content

extract

volatile oil were determined by using the method in the Chinese Pharmacopoeia of 2015 to evaluate the quality of medicinal materials from Chrysanthemi Flos of different regions. Random amplified polymorphic DNA (RAPD) molecular marker method was used to analyze the genetic relationship among Chrysanthemi Flos from different regions. Result: By analyzing the six indicatiors of Chrysanthemi Flos

its effective composition all conform the Chinese Pharmacopoeia of 2015. The results show that Wen county and Wuzhi county are suitable for the growth of Chrysanthemi Flos to be original regions. Seven RAPD primers had amplified into 50 clear bands including 46 polymorphic bands

so the polymorphism ratio was 92.00%. Huai Chrysanthemi Flos

Hang Chrysanthemi Flos

Gong Chrysanthemi Flos and Chrysanthemi Indici Flos can't be clustered

not only indicating that the genetic relationship among them was far

but also proving that abundant genetic diversity existed in the Chrysanthemi Flos germplasm resources. Huai Chrysanthemi Flos was clustered into a broad category

showing that the genetic purity of Huai Chrysanthemi Flos was higher. Conclusion: Analysis on Huai Chrysanthemi Flos germplasm resources

not only provided scientific reference basis for the standardization of Huai Chrysanthemi Flos planting

but also offered a scientific foundation for protection of Huai Chrysanthemi Flos resources and reasonable development and utilization of medicinal materials.