Objective: To separate and identify flavonoids extraction from Mori Cortex rapidly

and investigate their fragmentation behavior and secondary fragmentation pattern upon electrospray ionization. Method: The extraction was separated and identified inelectrospray ionization(ESI) negative mode by ultra performance liquid chromatography coupled with quadrupoletiem-of-flight mass spectrometry (UPLC-Q-TOF MS) technique.It was conducted on Waters BRH (2.1 mm×50 mm

1.7 μm) column with 0.1% formic acid solution (phase A) and acetonitrile (phase B) as the mobile phase for gradient elution.The column temperature was 25℃ and the flow rate was 0.3 mL·min-1. Result: Accurate relative molecular mass obtained by UPLC-Q-TOF-MS technique was combined with secondary mass spectrumfragmentation information for data statistics and analysis by using Masslynx 4.1.By referring to SciFinder database

13 flavonoids

including 7 Diels-Alder compounds (represented by sanggenon C and sanggenon D) and 6 prenyl flavonoids (represented by morusin)

were separated and identified from the flavonoids extraction of Mori Cortex. Conclusion: The loss of H2 and H2O was characterized here by using UPLC for the first time and their fragmentation behavior upon electrospray ionization was discussed. providinga rapid

simple and reliable method for the identification of flavonoids extraction from Mori Cortex.