Objective: To analyze and compare the headspace solid-phase microextraction methods (PA

PDMS

and PDMS-DVB) on adsorption property of volatile compounds in the dried whole plants of Hedyotis diffusa

and then compare them with traditional steam and distillation coupling with gas chromatography-mass spectrometry (SD-GC-MS). Method: Three types of SPME fibers including PDMS

PDMS-DVB and PA as well as steam distillation method were used for extraction

and GS-MS was combined for determination of the contents of volatile compounds. Result: The 50 compounds were identified by using PA method; 51 compounds were identified by using PDMS-DVB; 18 compounds were identified by using PDMS; and 41 components were identified by using SD-GC-MS. As compared with SPME method

PA method had the highest similarity

with 21 common components; followed by PDMS-DVB (15 common components) and PDMS (6 common components); 16 components were not identified by SPME method. Conclusion: HP-SPME-GC-MS is a simple

rapid and effective method for detecting the volatile components in H. diffusa

but cannot completely take the place of SD-GC-MS method.