Objective: To establish a targeted profiling qHNMR (TP-qHNMR) method for content determination of gentiopicroside and loganic acid in 4 kinds of Gentianae Macrophyllae Radix (Gentiana macrophμlla

G. straminea

G. crassicaulis and G. dahurica). Method: TP-qHNMR software of Chenomx NMR suit was employed and standard 1H-NMR spectra of gentiopicroside and loganic acid were collected. Gentianae Macrophyllae Radix was extracted by using methanol ultrasonic method and the dried extraction was reconstituted in CH3OD before spectra were recorded. All spectra were recorded in the same condition

and then targeted profiling quantification was achieved in reference to chemical shape indicator (CSI) by Chenomx profiler and further validated by HPLC-UV method. Result: The established TP-qHNMR method was accurate and reliable for determination of gentiopicroside and loganic acid in Gentianae Macrophyllae Radix

showing no significant difference with HPLC-UV method. The contents of gentiopicroside and loganic acid in 4 kinds of Gentianae Macrophyllae Radix were as follows:G. crassicaulis > G. macrophμlla > G. straminea > G. dahurica. Conclusion: TP-qHNMR was accurate and reproducibly for quality control of Gentianae Macrophyllae Radix

and can be widely applied for quality control of traditional Chinese medicine.