基于UPLC-Q-TOF/MS分析西洋参果总皂苷的入血成分

张楠淇; 李平亚; 王翠竹; 李卓; 王振洲; 崔涛; 刘金平

doi:10.13422/j.cnki.syfjx.2017060117

您当前的位置：

首页 >

文章列表页 >

基于UPLC-Q-TOF/MS分析西洋参果总皂苷的入血成分

药物代谢 | 更新时间：2024-01-04

- 基于UPLC-Q-TOF/MS分析西洋参果总皂苷的入血成分
- Absorbed Components Analysis of Total Saponins from Panacis Quinquefolii Radix Fruit in Rat Serum by UPLC-Q-TOF/MS
- 中国实验方剂学杂志 2017年23卷第6期页码：117-124
- 作者机构：
- 作者简介：
- 基金信息：
  
  吉林省科技发展计划重点科技成果转化项目（20140307005YY）
- DOI：10.13422/j.cnki.syfjx.2017060117
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
移动端阅览
张楠淇, 李平亚, 王翠竹, 等. 基于UPLC-Q-TOF/MS分析西洋参果总皂苷的入血成分[J]. 中国实验方剂学杂志, 2017,23(6):117-124.

ZHANG Nan-qi, LI Ping-ya, WANG Cui-zhu, et al. Absorbed Components Analysis of Total Saponins from Panacis Quinquefolii Radix Fruit in Rat Serum by UPLC-Q-TOF/MS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 117-124.
张楠淇, 李平亚, 王翠竹, 等. 基于UPLC-Q-TOF/MS分析西洋参果总皂苷的入血成分[J]. 中国实验方剂学杂志, 2017,23(6):117-124. DOI： 10.13422/j.cnki.syfjx.2017060117.

ZHANG Nan-qi, LI Ping-ya, WANG Cui-zhu, et al. Absorbed Components Analysis of Total Saponins from Panacis Quinquefolii Radix Fruit in Rat Serum by UPLC-Q-TOF/MS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 117-124. DOI： 10.13422/j.cnki.syfjx.2017060117.

摘要

目的：研究西洋参果总皂苷（TSPQF）灌胃给予大鼠后的入血成分，为其药效物质基础研究提供参考。方法：选取雄性Wistar大鼠为实验对象，以2.6 g·kg-1灌胃给予TSPQF，收集血清样品，采用超高效液相色谱-四级杆飞行时间质谱联用（UPLC-Q-TOF/MS）技术与多元统计分析相结合的方法快速分析并鉴定吸收入血的原型成分及其代谢产物。结果：大鼠含药血清中共鉴定了9个入血成分，其中5个入血成分为原型成分，分别为拟人参皂苷F11，人参皂苷Rc，Rb3，Rd和原人参三醇；其余4个为代谢产物，分别为原人参二醇，人参皂苷CK，人参皂苷Rh2和拟人参皂苷RT5。结论：9个入血成分可能是TSPQF在体内直接作用物质，为揭示TSPQF的药效物质基础提供了科学依据。

Abstract

Objective: To analyze and identify the constituents in rat serum after oral administration of total saponins from Panacis Quinquefolii Radix fruit (TSPQF)

and provide a reference for the pharmacodynamics material basis research. Method: The male Wistar rats were selected as experimental animals

rats were intragastric administered TSPQF at a dose of 2.6 g·kg-1.The serum samples were collected and pretreated.The absorbed prototype constituents and their metabolites in rat plasma were rapidly analyzed and identified by combination of UPLC-Q-TOF/MS and multivariate statistical analysis. Result: A total of 9 absorbed components were detected and identified

including 5 prototype components

such as pseudoginsenoside F11

ginsenoside Rc

Rb3

Rd and protopanaxatriol.The other 4 metabolites were protopanaxadiol

pseudoginsenoside RT5

ginsenoside CK

Rh2

respectively. Conclusion: These nine constituents absorbed into blood may be the potential bioactive components in TSPQF

this result can provide a scientific basis for clarifying its substance basis of pharmacodynamics.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

接种解有机磷细菌对滇重楼品质及土壤肥力的影响

不同丛枝菌根真菌组合对滇重楼光合生理和化学成分的影响

不同丛枝菌根真菌对滇重楼幼苗根际土壤养分的影响

多指标正交试验优选升麻提取工艺

柴胡总皂苷提取物制剂前的评价