Objective: To establish a migration platform for neural stem cells(NSCs) in vitro and verify the applicability of this platform by using Buyang Huanwu Tang

a Chinese classical formula. Method: NSCs were isolated from rat embryos

and used in establishing a migration platform for neural stem cells in vitro by radial cell migration assay

scratch repair dynamic detection

and Transwell chemotaxis system. Then this platform was used to evaluate the effects ofof Buyang Huanwu Tang(300

600 mg·L-1)

Tetramethylpyrazine (10

50 mg·L-1)

as well as stromal-derived factor 1(SDF-1) on NSCs migration. ELISA assay was used to detect the contents of migration regulatory factor SDF-1 and vascular endothelial growth factor(VEGF) in the radial cell migration and transwell system. Result: NSCs migration in varying degrees was observed at different time points in radial migration

scratch repair dynamic detection and transwell chemotaxis system. As compared with the blank group

Buyang Huanwu Tang and Tetramethylpyrazine could significantly promote NSCs migration in radio migration system

but also significantly increase the number of NSCs migrated to lower chamber in Transwell system (P<0.05

P<0.01) in a dose-dependent manner. Additionally

Buyang Huanwu Tang and Tetramethylpyrazine could promote the expression levels of SDF-1 and VEGF in NSCs culture supernatant (P<0.01)

where the up-regulation effect on SDF-1 was higher than that on VEGF. We also found that the effect of Buyang Huanwu Tang (600 mg·L-1) and Tetramethylpyrazine (10

50 mg·L-1) were inhibited after AMD3100 pretreatment

where the number of NSCs migrated to lower champ was reduced significantly. Conclusion: The NSCs migration research platform established in this study is dynamic and multi-dimensional. It can simulate different clinical pathophysiological processes

and in addition

this platform is economical

practical and easy to operate.