桑叶对糖尿病小鼠肝脏Toll样受体基因表达的影响

田思敏; 柳辰玥; 马双双; 张弛; 赵保胜

doi:10.13422/j.cnki.syfjx.2017060137

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桑叶对糖尿病小鼠肝脏Toll样受体基因表达的影响

药理 | 更新时间：2024-01-04

- 桑叶对糖尿病小鼠肝脏Toll样受体基因表达的影响
- Effect of Mori Folium on Gene Expression of TLRs in Liver of Diabetic Mice
- 中国实验方剂学杂志 2017年23卷第6期页码：137-142
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81374027）
- DOI：10.13422/j.cnki.syfjx.2017060137
  中图分类号： R285.5
- 纸质出版日期：2017
- 稿件说明：
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田思敏, 柳辰玥, 马双双, 等. 桑叶对糖尿病小鼠肝脏Toll样受体基因表达的影响[J]. 中国实验方剂学杂志, 2017,23(6):137-142.

TIAN Si-min, LIU Chen-yue, MA Shuang-shuang, et al. Effect of Mori Folium on Gene Expression of TLRs in Liver of Diabetic Mice[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 137-142.
田思敏, 柳辰玥, 马双双, 等. 桑叶对糖尿病小鼠肝脏Toll样受体基因表达的影响[J]. 中国实验方剂学杂志, 2017,23(6):137-142. DOI： 10.13422/j.cnki.syfjx.2017060137.

TIAN Si-min, LIU Chen-yue, MA Shuang-shuang, et al. Effect of Mori Folium on Gene Expression of TLRs in Liver of Diabetic Mice[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 137-142. DOI： 10.13422/j.cnki.syfjx.2017060137.

摘要

目的：探讨桑叶水提物对糖尿病小鼠肝组织Toll样受体（Toll-like receptors，TLRs）基因表达量的影响，并探讨其相关的作用机制。方法：ICR小鼠随机分为正常组（6只），糖尿病组。糖尿病组小鼠利用高脂高糖饲料喂养10周联合多次注射链脲佐菌素（STZ）建立高脂饲养和STZ共同诱导的小鼠糖尿病模型。造模成功小鼠随机分为模型组，二甲双胍组（600 mg·kg-1），桑叶高、中、低剂量组（8，4，2 g·kg-1），每组6只，灌胃（ig）给药，每周测空腹血糖，于实验中后期行口服糖耐量实验，ig给药10周后，测定小鼠血清胰岛素、总胆固醇、甘油三酯和肿瘤坏死因子-α（TNF-α），并应用实时荧光定量PCR（Real-time PCR）检测小鼠肝组织中TLR1~9表达量。结果：糖尿病模型小鼠经桑叶水提物治疗后，进水量减少，显著降低空腹血糖，改善糖耐量，显著降低血脂，增加胰岛素含量，改善胰岛素抵抗，同时降低血清中TNF-α（P<0.05，P<0.01）。Real-time PCR结果显示，糖尿病小鼠肝脏组织TLRs受体中TLR1，TLR2，TLR3，TLR7，TLR8和TLR9表达明显高于正常组（P<0.05，P<0.01），TLR5表达明显低于正常组；桑叶给药组后，桑叶中剂量组肝组织中的TLR7，TLR8和TLR9表达量，显著降低于模型组（P<0.05，P<0.01）。结论：桑叶水提物的降糖作用可能与抑制糖尿病模型小鼠肝组织中TLRs受体有关。

Abstract

Objective: To investigate the regulatory effect of the water extract from Mori Folium on the gene expressions of Toll-like receptors (TLRs) in the liver from diabetic mice

and explore its action mechanism. Method: ICR mice were randomly divided into normal group (n=6) and diabetic group. The mice in diabetic group received high-fat and high-sugar fodder for 10 weeks and injection with 100 mg·kg-1 streptozotocin (STZ) for 3 days continuously to establish the diabetic mice models. The successfully modeled mice were then randomly divided into model group

metformin gorup (600 mg·kg-1)

Mori Folium groups (high dose 8 g·kg-1

middle dose 4 g·kg-1

and low dose 2 g·kg-1) for 10 weeks

n=6 in each group. The drugs were given by ig administration

and the fasting blood glucose was detected every week. Oral glucose tolerance test was conducted in the mid and later phases of experiment. After ig administration for 10 weeks

serum insulin (INS)

total cholesterol (T-CHO)

triglyceride (TG) and tumor necrosis factor-α (TNF-α) were detected

and the expression level of TLR 1-9 in the liver was measured with quantitative real-time PCR. Result: After the diabetic model mice were treated with Mori Folium water extract

their water intake was reduced; fasting blood glucose was significantly reduced; glucose tolerance was improved; blood fat was significantly reduced; insulin concentration was dramatically increased; insulin resistance was improved; and serum TNF-α level was reduced (P<0.05

P<0.01). Quantitative Real-time PCR data showed that TLR1

TLR2

TLR3

TLR7

TLR8 and TLR9 mRNA expression levels were significantly increased in livers of the diabetic rats (P<0.05

P<0.01

as compared with normal group)

and TLR5 expression level in model group was significantly lower than that in normal group. In addition

the expression levels of TLR7

TLR8 and TLR9 in liver of Mori Folium middle dose group were significantly lower than those in model group (P<0.05

P<0.01). Conclusion: The water extract of Mori Folium could ameliorate diabetes

and its mechanism may be partly related to inhibiting the TLRs expression in the liver.

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