Objective: To study the anti-tumor effect of astragaloside combined with curcumin on human ovarian cancer HO-8910 orthotopic transplantation tumor metastasis and discuss whether the combination of astragalosideand curcumin has the synergies effect against tumor. Method: HO-8910 ovarian carcinoma animal models withfluorescent protein transfectionwere selected

including G1 model group

G2 Cisplatin group

G3 Astragaloside group

G4 curcumin group

G5 Astragaloside+curcumin group

n=8 in each group. The tumor was weighted and the inhibitory rate was calculated. The protein expression levelsof matrix metallopeptidase 2 (MMP2) and apoptosis regulator (Bcl-2) were detected by immunohistochemical method

and thegene expression levels of MMP2

Bcl-2

miR21

miR15a and miR200a were detected by Real-time PCR. Result: Tumor weights of the tumor-bearing nude mice after treatment were lower than those in model group; the tumor weight in Astragaloside+curcumin group was significantly lower than that in other groups(P<0.05).Immunohistochemical results showed that the levels of the protein expressions of MMP2

Bcl-2 in G2

G3 and G4 groups were lower than those in model group

and the levels in G5 group were significantly reduced (P<0.05). Real-time PCR results showed that the gene expression levels of MMP2

Bcl-2 and miR21 in Chinese medicine groups were lower than those in model group

and the levels in G5 groupweresignificantly reduced (P<0.05); the gene expression levels of miR15a

miR200a were higher than those in model group

and the levels in G5 group were significantly increased (P<0.05). Conclusion: The combination of astragaloside and curcumin has the suppression effect on human ovarian cancer HO-8910 orthotopic transplantation tumor metastasis

which may be associated with the down-regulation of MMP2

Bcl-2 and miR21 expressions and the up-regulation of miR15a and miR200a expressions. Therefore

the combination of astragaloside and curcumin has the synergies effect against tumor.